phosphorus-radioisotopes and phenylisothiocyanate

phosphorus-radioisotopes has been researched along with phenylisothiocyanate* in 1 studies

Other Studies

1 other study(ies) available for phosphorus-radioisotopes and phenylisothiocyanate

Article	Year
Determination of phosphorylation sites in peptides and proteins employing a volatile Edman reagent. Journal of protein chemistry, 1997, Volume: 16, Issue:5 A manual Edman degradation protocol has been developed that allows the identification of phosphorylation sites in 32P-labeled peptides at the subpicomole level. By using both a volatile reagent, trifluoroethyl isothiocyanate, and volatile buffers, extraction steps are rendered unnecessary and cycle times can be reduced to 45 min. The protocol was employed to identify the site of phosphorylation in phosphoserine- and phosphotyrosine-containing peptides. Topics: Binding Sites; Chromatography, Thin Layer; Electrophoresis; Humans; Isothiocyanates; Peptides; Phosphorus Radioisotopes; Phosphorylation; Proteins; Thiocyanates	1997

Article

Year

Determination of phosphorylation sites in peptides and proteins employing a volatile Edman reagent.

Journal of protein chemistry, 1997, Volume: 16, Issue:5

A manual Edman degradation protocol has been developed that allows the identification of phosphorylation sites in 32P-labeled peptides at the subpicomole level. By using both a volatile reagent, trifluoroethyl isothiocyanate, and volatile buffers, extraction steps are rendered unnecessary and cycle times can be reduced to 45 min. The protocol was employed to identify the site of phosphorylation in phosphoserine- and phosphotyrosine-containing peptides.

Topics: Binding Sites; Chromatography, Thin Layer; Electrophoresis; Humans; Isothiocyanates; Peptides; Phosphorus Radioisotopes; Phosphorylation; Proteins; Thiocyanates

1997