phosphorus-radioisotopes and inositol-1-2-cyclic-4-5-triphosphate

phosphorus-radioisotopes has been researched along with inositol-1-2-cyclic-4-5-triphosphate* in 1 studies

Other Studies

1 other study(ies) available for phosphorus-radioisotopes and inositol-1-2-cyclic-4-5-triphosphate

ArticleYear
Synthesis of inositol 1,2-(cyclic)-4,5-trisphosphate.
    Proceedings of the National Academy of Sciences of the United States of America, 1987, Volume: 84, Issue:5

    We have developed a method for synthesis of inositol 1,2-(cyclic)-4,5-trisphosphate from inositol 1,4,5-trisphosphate using a water-soluble carbodiimide. We obtained 1-1.5 mumol of the inositol cyclic trisphosphate starting with 5 mumol of inositol 1,4,5-trisphosphate. The cyclized product was isolated by HPLC on Partisil SAX. The identity of the cyclic product was verified by its hydrolysis to inositol 1,4,5-trisphosphate in acid and by its conversion to 1,2-(cyclic)-4-bisphosphate by a specific 5-phosphomonoesterase from platelets. We also identified the product by 31P NMR spectroscopy, which showed a peak at 17.2 ppm, characteristic of a five-membered cyclic phosphodiester ring, and peaks at 4.1 ppm and 0.8 ppm, indicative of phosphomonoesters. This relatively simple method for producing inositol 1,2-(cyclic)-4,5-trisphosphate will facilitate studies of the physiology of this compound in signal transduction.

    Topics: Chromatography, High Pressure Liquid; Indicators and Reagents; Inositol Phosphates; Magnetic Resonance Spectroscopy; Phosphorus Radioisotopes; Sugar Phosphates

1987
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