phosphorus-radioisotopes and dipalmitoylphosphatidic-acid

phosphorus-radioisotopes has been researched along with dipalmitoylphosphatidic-acid* in 1 studies

Other Studies

1 other study(ies) available for phosphorus-radioisotopes and dipalmitoylphosphatidic-acid

Article	Year
A procedure for the preparation of [32P]phosphatidic acid. Analytical biochemistry, 1986, May-15, Volume: 155, Issue:1 The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in 2 h. The product was shown to be radiochemically pure by both TLC and HPLC. L- and DL-[32P]dipalmitoyl phosphatidic acid prepared using this procedure were shown to be hydrolyzed by rat liver microsomes at approximately the same rates. Topics: Animals; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Hydrolysis; In Vitro Techniques; Isotope Labeling; Microsomes, Liver; Phosphatidic Acids; Phosphorus Radioisotopes; Phosphorylation; Rats; Stereoisomerism	1986

Article

Year

A procedure for the preparation of [32P]phosphatidic acid.

Analytical biochemistry, 1986, May-15, Volume: 155, Issue:1

The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in 2 h. The product was shown to be radiochemically pure by both TLC and HPLC. L- and DL-[32P]dipalmitoyl phosphatidic acid prepared using this procedure were shown to be hydrolyzed by rat liver microsomes at approximately the same rates.

Topics: Animals; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Hydrolysis; In Vitro Techniques; Isotope Labeling; Microsomes, Liver; Phosphatidic Acids; Phosphorus Radioisotopes; Phosphorylation; Rats; Stereoisomerism

1986