phosphorus-radioisotopes and dimethyl-methylphosphonate

phosphorus-radioisotopes has been researched along with dimethyl-methylphosphonate* in 2 studies

Other Studies

2 other study(ies) available for phosphorus-radioisotopes and dimethyl-methylphosphonate

Article	Year
31P MAS-NMR of human erythrocytes: independence of cell volume from angular velocity. Magnetic resonance in medicine, 2004, Volume: 52, Issue:3 31P magic angle spinning NMR (MAS-NMR) spectra were obtained from suspensions of human red blood cells (RBCs) that contained the cell-volume-sensitive probe molecule, dimethyl methylphosphonate (DMMP). A mathematical representation of the spectral-peak shape, including the separation and width-at-half-height in the 31P NMR spectra, as a function of rotor speed, enabled us to explore the extent to which a change in cell volume would be reflected in the spectra if it occurred. We concluded that a fractional volume change in excess of 3% would have been detected by our experiments. Thus, the experiments indicated that the mean cell volume did not change by this amount even at the highest spinning rate of 7 kHz. The mean cell volume and intracellular 31P line-width were independent of the packing density of the cells and of the initial cell volume. The relationship of these conclusions to other non-NMR studies of pressure effects on cells is noted. Topics: Computer Simulation; Erythrocyte Volume; Humans; Magnetic Resonance Spectroscopy; Organophosphorus Compounds; Phosphorus Radioisotopes; Pressure; Temperature	2004
Further investigation of the use of dimethyl methylphosphonate as a 31P-NMR probe of red cell volume. Biochimica et biophysica acta, 1988, Feb-22, Volume: 968, Issue:2 We have refined a method for measuring erythrocyte volume using the 31P-NMR spectrum of a probe molecule, dimethyl methylphosphonate. This compound, when added to an erythrocyte suspension, gives rise to two 31P-NMR resonances, and the frequency separation between them is linearly dependent on the intracellular haemoglobin concentration. If, for a given cell sample (under standard conditions), the separation of the two dimethyl methylphosphonate peaks has been measured and an independent estimation of the mean cell haemoglobin content and concentration has been obtained, then changes in the mean cell volume due to altered experimental conditions may be estimated from the peak separation measured under the new conditions. Although the peak separation was independent of extracellular pH, it did vary with (i) a range of extracellular suspension media, (ii) temperature, (iii) dimethyl methylphosphonate concentration, (iv) haemoglobin ligand state and (v) different blood donors. Topics: Carboxyhemoglobin; Erythrocyte Volume; Hemoglobinometry; Humans; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Organophosphorus Compounds; Oxyhemoglobins; Phosphorus Radioisotopes; Temperature	1988

Article

Year

31P MAS-NMR of human erythrocytes: independence of cell volume from angular velocity.

Magnetic resonance in medicine, 2004, Volume: 52, Issue:3

31P magic angle spinning NMR (MAS-NMR) spectra were obtained from suspensions of human red blood cells (RBCs) that contained the cell-volume-sensitive probe molecule, dimethyl methylphosphonate (DMMP). A mathematical representation of the spectral-peak shape, including the separation and width-at-half-height in the 31P NMR spectra, as a function of rotor speed, enabled us to explore the extent to which a change in cell volume would be reflected in the spectra if it occurred. We concluded that a fractional volume change in excess of 3% would have been detected by our experiments. Thus, the experiments indicated that the mean cell volume did not change by this amount even at the highest spinning rate of 7 kHz. The mean cell volume and intracellular 31P line-width were independent of the packing density of the cells and of the initial cell volume. The relationship of these conclusions to other non-NMR studies of pressure effects on cells is noted.

Topics: Computer Simulation; Erythrocyte Volume; Humans; Magnetic Resonance Spectroscopy; Organophosphorus Compounds; Phosphorus Radioisotopes; Pressure; Temperature

2004

Further investigation of the use of dimethyl methylphosphonate as a 31P-NMR probe of red cell volume.

Biochimica et biophysica acta, 1988, Feb-22, Volume: 968, Issue:2

We have refined a method for measuring erythrocyte volume using the 31P-NMR spectrum of a probe molecule, dimethyl methylphosphonate. This compound, when added to an erythrocyte suspension, gives rise to two 31P-NMR resonances, and the frequency separation between them is linearly dependent on the intracellular haemoglobin concentration. If, for a given cell sample (under standard conditions), the separation of the two dimethyl methylphosphonate peaks has been measured and an independent estimation of the mean cell haemoglobin content and concentration has been obtained, then changes in the mean cell volume due to altered experimental conditions may be estimated from the peak separation measured under the new conditions. Although the peak separation was independent of extracellular pH, it did vary with (i) a range of extracellular suspension media, (ii) temperature, (iii) dimethyl methylphosphonate concentration, (iv) haemoglobin ligand state and (v) different blood donors.

Topics: Carboxyhemoglobin; Erythrocyte Volume; Hemoglobinometry; Humans; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Organophosphorus Compounds; Oxyhemoglobins; Phosphorus Radioisotopes; Temperature

1988