phosphorus-radioisotopes has been researched along with 4-bromotetramisole* in 2 studies
2 other study(ies) available for phosphorus-radioisotopes and 4-bromotetramisole
| Article | Year |
|---|---|
Stabilization of phospho-intermediates of rat liver plasma membrane alkaline phosphatase by uncompetitive inhibition. Relation with phosphate uptake into hepatocytes.
Rat liver plasma membrane alkaline phosphatase (ALP) phospho-intermediates, which have molecular masses of 151 and 135 kDa bands, were labelled at physiological pH with either (gamma-32P) ATP or 32Pi. This labeling was stabilized by a potent enzyme inhibitor, bromolevamisole (BL), and not by bromodexamisole (BD). BL augmented the rate and extent of autophosphorylation and slowed down the rate of autodephosphorylation of ALP. The phospho-intermediates labeling presented nearly the same kinetic behaviour with either (gamma-32P) ATP or 32Pi. In the presence of BL a marked decrease of the phosphorylation state of many proteins was observed in hepatocytes. BL also produced a decrease of the 32Pi uptake into hepatocytes and a decrease of the specific radioactivity of cellular ATP. BD had nearly the same effect as BL on protein phosphorylation and 32Pi uptake. These results argued against a direct involvement of ALP in Pi transport across hepatocyte plasma membrane. Topics: Adenosine Triphosphate; Alkaline Phosphatase; Animals; Biological Transport; Cell Membrane; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Kinetics; Levamisole; Liver; Male; Phosphates; Phosphoproteins; Phosphorus Radioisotopes; Rats; Rats, Wistar; Tetramisole; Time Factors | 1993 |
Effect of alkaline-phosphatase inhibition by 1-p-bromotetramisole on the formation of trichloroacetic acid-[32P]-insoluble phosphate from inorganic [32P]-phosphate and [32P]-pyrophosphate in non-mineralizing and mineralizing hamster molar tooth-germs in v
In culture, 1-p-bromotetramisole (pBTM), a specific inhibitor of alkaline phosphatase, significantly inhibited the formation of trichloroacetic acid (TCA)-insoluble [32P]-phosphate from inorganic [32P]-phosphate in the proliferating non-mineralizing second (M2) maxillary molar germs but had no effect in the actively mineralizing first (M1) germs. Addition of 10(-5) M inorganic pyrophosphate in the culture medium with a [32P]-phosphate label increased the inhibition of the formation of TCA-insoluble [32P]-phosphate in the M2. pBTM almost completely inhibited the formation of TCA-insoluble [32P]-phosphate from inorganic [32P]-pyrophosphate in the non-mineralizing M2. In the actively mineralizing M1, the compound significantly inhibited but did not abolish the formation of TCA-insoluble phosphate. These results confirm earlier biochemical findings that alkaline phosphatase possesses a pyrophosphatase activity probably related to the turnover of phosphorylated macromolecules necessary for cell differentiation and proliferation. Topics: Alkaline Phosphatase; Animals; Cricetinae; Diphosphates; Mesocricetus; Molar; Phosphates; Phosphorus Radioisotopes; Tetramisole; Time Factors; Tooth Calcification; Tooth Germ; Trichloroacetic Acid | 1987 |