phosphoramidon and hemorphin-4

In these experiments we have studied the in vitro metabolism of LVV-hemorphin-7 in human plasma by using reversed-phase high-performance liquid chromatography (RP-HPLC) in combination with micro-electrospray mass spectrometry (micro-ES-MS). Tandem mass spectrometry (MS-MS) was performed in order to verify the structure of the peptide fragments found. Incubations were performed with and without different protease inhibitors. Results showed that LVV-hemorphin-7 was metabolized from the N-terminal end of the peptide, probably by an amastatin-sensitive exopeptidase.

Topics: Amino Acid Sequence; Angiotensin-Converting Enzyme Inhibitors; Anti-Bacterial Agents; Captopril; Chromatography, High Pressure Liquid; Endorphins; Gas Chromatography-Mass Spectrometry; Glycopeptides; Hemoglobins; Humans; Molecular Sequence Data; Opioid Peptides; Peptide Fragments; Peptides; Plasma; Protease Inhibitors; Spectrophotometry, Ultraviolet; Time Factors