phosphoramidite and thymine-arabinoside

phosphoramidite has been researched along with thymine-arabinoside* in 1 studies

Other Studies

1 other study(ies) available for phosphoramidite and thymine-arabinoside

Article	Year
Synthesis and incorporation of an alpha-hexofuranosyl thymidine into oligodeoxynucleotides via its two exocyclic OH-groups. Bioorganic & medicinal chemistry letters, 2004, Feb-09, Volume: 14, Issue:3 1-(2,3-Dideoxy-3-amino-alpha-D-arabino-hexofuranosyl)thymine is considered as a conformationally restricted acyclic nucleoside using the furanose ring to link the diol backbone to the nucleobase. The appropriately substituted phosphoramidites were synthesised via 1-(5,6-di-O-acetyl-2,3-dideoxy-3-phthalimido-alpha-D-arabino-hexofuranosyl)thymine and used in oligodeoxynucleotide (ODN) synthesis. However, the binding affinity of the mixed ODNs towards complementary DNA and RNA was decreased compared to the wild-type oligos. The decrease was smaller when the monomer was inserted near the end of the sequence. The insertions into an alpha T sequence or in a beta T sequence gave nearly the same dropping in melting temperature per modification which indicates that the new nucleotide modifications behave both as alpha and beta nucleotides. Topics: Arabinonucleosides; Binding Sites; DNA, Complementary; Hydroxides; Molecular Structure; Oligodeoxyribonucleotides; Organophosphorus Compounds; RNA; Temperature; Thymidine	2004

Article

Year

Synthesis and incorporation of an alpha-hexofuranosyl thymidine into oligodeoxynucleotides via its two exocyclic OH-groups.

Bioorganic & medicinal chemistry letters, 2004, Feb-09, Volume: 14, Issue:3

1-(2,3-Dideoxy-3-amino-alpha-D-arabino-hexofuranosyl)thymine is considered as a conformationally restricted acyclic nucleoside using the furanose ring to link the diol backbone to the nucleobase. The appropriately substituted phosphoramidites were synthesised via 1-(5,6-di-O-acetyl-2,3-dideoxy-3-phthalimido-alpha-D-arabino-hexofuranosyl)thymine and used in oligodeoxynucleotide (ODN) synthesis. However, the binding affinity of the mixed ODNs towards complementary DNA and RNA was decreased compared to the wild-type oligos. The decrease was smaller when the monomer was inserted near the end of the sequence. The insertions into an alpha T sequence or in a beta T sequence gave nearly the same dropping in melting temperature per modification which indicates that the new nucleotide modifications behave both as alpha and beta nucleotides.

Topics: Arabinonucleosides; Binding Sites; DNA, Complementary; Hydroxides; Molecular Structure; Oligodeoxyribonucleotides; Organophosphorus Compounds; RNA; Temperature; Thymidine

2004