phosphoramidite and butane

phosphoramidite has been researched along with butane* in 1 studies

Other Studies

1 other study(ies) available for phosphoramidite and butane

Article	Year
Design, synthesis, biophysical and primer extension studies of novel acyclic butyl nucleic acid (BuNA). Organic & biomolecular chemistry, 2013, Sep-21, Volume: 11, Issue:35 A novel nucleic acid analogue called acyclic (S)-butyl nucleic acid (BuNA) composed of an acyclic backbone containing a phosphodiester linkage and bearing natural nucleobases was synthesized. Next, (S)-BuNA nucleotides were incorporated in DNA strands and their effect on duplex stability and changes in structural conformation were investigated. Circular dichroism (CD), UV-melting and non-denatured gel electrophoresis (native PAGE) studies revealed that (S)-BuNA is capable of making duplexes with its complementary strands and integration of (S)-BuNA nucleotides into DNA duplex does not alter the B-type-helical structure of the duplex. Furthermore, (S)-BuNA oligonucleotides and (S)-BuNA substituted DNA strands were studied as primer extensions by DNA polymerases. This study revealed that the acyclic scaffold is tolerated by enzymes and is therefore to some extent biocompatible. Topics: Base Pairing; Base Sequence; Butanes; DNA; Nucleic Acid Conformation; Nucleic Acids; Oligonucleotides; Organophosphorus Compounds	2013

Article

Year

Design, synthesis, biophysical and primer extension studies of novel acyclic butyl nucleic acid (BuNA).

Organic & biomolecular chemistry, 2013, Sep-21, Volume: 11, Issue:35

A novel nucleic acid analogue called acyclic (S)-butyl nucleic acid (BuNA) composed of an acyclic backbone containing a phosphodiester linkage and bearing natural nucleobases was synthesized. Next, (S)-BuNA nucleotides were incorporated in DNA strands and their effect on duplex stability and changes in structural conformation were investigated. Circular dichroism (CD), UV-melting and non-denatured gel electrophoresis (native PAGE) studies revealed that (S)-BuNA is capable of making duplexes with its complementary strands and integration of (S)-BuNA nucleotides into DNA duplex does not alter the B-type-helical structure of the duplex. Furthermore, (S)-BuNA oligonucleotides and (S)-BuNA substituted DNA strands were studied as primer extensions by DNA polymerases. This study revealed that the acyclic scaffold is tolerated by enzymes and is therefore to some extent biocompatible.

Topics: Base Pairing; Base Sequence; Butanes; DNA; Nucleic Acid Conformation; Nucleic Acids; Oligonucleotides; Organophosphorus Compounds

2013