phosphoramidite has been researched along with acetonitrile* in 3 studies
3 other study(ies) available for phosphoramidite and acetonitrile
| Article | Year |
|---|---|
Accurate mass analysis of phosphoramidites by electrospray mass spectrometry.
A method of accurate mass determination of phosphoramidites is described. The commonly used methanol/water/acid system was replaced by LiCl-containing acetonitrile and the concentrations of LiCl, poly(ethylene glycol), and phosphoramidite samples were optimized. Topics: Acetonitriles; Lithium Chloride; Macromolecular Substances; Mass Spectrometry; Models, Chemical; Molecular Biology; Organophosphorus Compounds; Polyethylene Glycols; Polymers; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization | 2005 |
Solution stability and degradation pathway of deoxyribonucleoside phosphoramidites in acetonitrile.
The impuritiy profiles of acetonitrile solutions of the four standard O-cyanoethyl-N,N-diisopropyl-phosphoramidites of 5'-O-dimethoxytrityl (DMT) protected deoxyribonucleosides (dG(ib), dA(bz), dC(bz), T) were analyzed by HPLC-MS. The solution stability of the phosphoramidites decreases in the order T, dC>dA>dG. After five weeks storage under inert gas atmosphere the amidite purity was reduced by 2% (T, dC), 6% (dA), and 39% (dG), respectively. The main degradation pathways involve hydrolysis, elimination of acrylonitrile and autocatalytic acrylonitrile-induced formation of cyanoethyl phosphonoamidates. Consequently, the rate of degradation is reduced by reducing the water concentration in solution with molecular sieves and by lowering the amidite concentration. Acid-catalyzed hydrolysis could also be reduced by addition of small amounts of base. Topics: Acetonitriles; Deoxyribonucleosides; Organophosphorus Compounds; Solutions | 2004 |
Design, synthesis, and amplification of DNA pools for in vitro selection.
Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions. Topics: Acetonitriles; Base Sequence; DNA; Molecular Sequence Data; Molecular Weight; Organophosphorus Compounds; Polymerase Chain Reaction; SELEX Aptamer Technique | 2001 |