phosphoramidite and 2-amino-3-methylimidazo(4-5-f)quinoline

phosphoramidite has been researched along with 2-amino-3-methylimidazo(4-5-f)quinoline* in 1 studies

Other Studies

1 other study(ies) available for phosphoramidite and 2-amino-3-methylimidazo(4-5-f)quinoline

Article	Year
Synthesis of oligonucleotides containing the N2-deoxyguanosine adduct of the dietary carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline. Chemical research in toxicology, 2007, Volume: 20, Issue:12 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) is a highly mutagenic heterocyclic amine formed in all cooked meats. IQ has been found to be a potent inducer of frameshift mutations in bacteria and carcinogenic in laboratory animals. Upon metabolic activation, IQ forms covalent adducts at the C8- and N2-positions of deoxyguanosine with a relative ratio of up to approximately 4:1. We have previously incorporated the major dGuo-C8-IQ adduct into oligonucleotides through the corresponding phosphoramidite reagent. We report here the sequence-specific synthesis of oligonucleotides containing the minor dGuo-N2-IQ adduct. Thermal melting analysis revealed that the dGuo-N2-IQ adduct significantly destabilizes duplex DNA. Topics: Carcinogens; Circular Dichroism; Deoxyguanosine; Electrophoresis, Capillary; Oligonucleotides; Organophosphorus Compounds; Quinolines	2007

Article

Year

Synthesis of oligonucleotides containing the N2-deoxyguanosine adduct of the dietary carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline.

Chemical research in toxicology, 2007, Volume: 20, Issue:12

2-amino-3-methylimidazo[4,5-f]quinoline (IQ) is a highly mutagenic heterocyclic amine formed in all cooked meats. IQ has been found to be a potent inducer of frameshift mutations in bacteria and carcinogenic in laboratory animals. Upon metabolic activation, IQ forms covalent adducts at the C8- and N2-positions of deoxyguanosine with a relative ratio of up to approximately 4:1. We have previously incorporated the major dGuo-C8-IQ adduct into oligonucleotides through the corresponding phosphoramidite reagent. We report here the sequence-specific synthesis of oligonucleotides containing the minor dGuo-N2-IQ adduct. Thermal melting analysis revealed that the dGuo-N2-IQ adduct significantly destabilizes duplex DNA.

Topics: Carcinogens; Circular Dichroism; Deoxyguanosine; Electrophoresis, Capillary; Oligonucleotides; Organophosphorus Compounds; Quinolines

2007