phosphoramidite and 1-ethynylpyrene

phosphoramidite has been researched along with 1-ethynylpyrene* in 1 studies

Other Studies

1 other study(ies) available for phosphoramidite and 1-ethynylpyrene

Article	Year
Synthesis of pyrene labeled RNA for fluorescence measurements. Nucleosides, nucleotides & nucleic acids, 2007, Volume: 26, Issue:10-12 The fluorophores 1-ethynylpyrene and 1-(p-ethynyl-phenylethynyl)-pyrene were attached to RNA through a Sonogashira cross-coupling with 5-iodocytidine either in solution through phosphoamidite synthesis or via on-column conjugation during solid-phase oligonucleotide synthesis. Six probes with the sequence 5'-CUU UUC UUU CUU-3' were derivatized with both fluorophores, whereby the position of the modified cytidine was varied. Fluorescence measurements showed sensitivity of the pyrene group to its environment in the single strands and corresponding duplexes. Topics: Fluorescence; Oligonucleotides; Organophosphorus Compounds; Pyrenes; RNA; RNA Probes; Spectrometry, Fluorescence	2007

Article

Year

Synthesis of pyrene labeled RNA for fluorescence measurements.

Nucleosides, nucleotides & nucleic acids, 2007, Volume: 26, Issue:10-12

The fluorophores 1-ethynylpyrene and 1-(p-ethynyl-phenylethynyl)-pyrene were attached to RNA through a Sonogashira cross-coupling with 5-iodocytidine either in solution through phosphoamidite synthesis or via on-column conjugation during solid-phase oligonucleotide synthesis. Six probes with the sequence 5'-CUU UUC UUU CUU-3' were derivatized with both fluorophores, whereby the position of the modified cytidine was varied. Fluorescence measurements showed sensitivity of the pyrene group to its environment in the single strands and corresponding duplexes.

Topics: Fluorescence; Oligonucleotides; Organophosphorus Compounds; Pyrenes; RNA; RNA Probes; Spectrometry, Fluorescence

2007