pheophytin-a and arsenic-acid

Hydrilla verticillata (L.f.) Royle has a great ability to accumulate large amounts of arsenic (As). We studied the influence of phosphorus (P), nitrogen (N), pH, and arsenite (As(III)) on As transformation and fate in the H. verticillata - medium system via orthogonal experimental design. The results showed highest plant growth was under intermediate As(III) in the medium, with Chlorophyll a and Chlorophyll b contents in plant diminishing after 96 h treatment. Exposure to high N, high As(III), intermediate P, and low pH in the medium, the highest total arsenic uptake by plants were 169.1 ± 5.5 μg g

Topics: Arsenates; Arsenic; Arsenites; Biodegradation, Environmental; Chlorophyll; Chlorophyll A; Hydrocharitaceae; Oxidation-Reduction

Cytokinins (CTKs) are effective in alleviating abiotic stresses on plants, but little information is available regarding the effects of CTKs on arsenic (As) accumulation and changes of chloroplast ultrastructure in plants with different As-accumulating ability. Here a hydroponic experiment was designed to evaluate the effects of different concentration of kinetin (KT, 0-40 mg/L) on growth and chloroplast ultrastructure of As hyperaccumulator Pteris cretica var. nervosa and non-hyperaccumulator Pteris ensiformis treated by 5 mg/L arsenate for 14 days. The growth parameters, As accumulation, contents of photosynthetic pigments and chloroplast ultrastructure were examined. The results showed that KT promoted the growth of two plants, and significantly increased As accumulation and translocation in P. cretica var. nervosa and P. ensiformis at 5 and 20 mg/L, respectively. Additionally, the contents of chlorophyll a and carotenoid in two plants showed no significant difference at 20 mg/L KT compared to the control. Chloroplast ultrastructure of P. cretica var. nervosa was integral with KT application. Comparatively, the swollen chloroplasts were increased, plasmolysis appeared, and chloroplast grana slice layers and stroma lamellas were clearly separated or distorted at 5 mg/L KT in P. ensiformis. The length and width of chloroplasts in P. cretica var. nervosa were significantly increased with KT addition compared to the control. However, the length of chloroplasts in P. ensiformis was significantly decreased but their width showed no significant change. Furthermore, the deterioration of chloroplast ultrastructure in P. ensiformis was ameliorated by 40 mg/L KT. These results suggested that KT increased As accumulation and was beneficial to maintain the photosynthetic pigments for a good growth of plants. Therefore, KT could maintain and reorganize the ultrastructure integrality of As-stressed chloroplasts to some extent for the two plants, especially at high concentration.

Topics: Adaptation, Physiological; Arsenates; Arsenic; Carotenoids; Chlorophyll; Chlorophyll A; Chloroplasts; Hydroponics; Kinetin; Photosynthesis; Plant Development; Plant Growth Regulators; Pteris; Species Specificity; Stress, Physiological

The metalloid arsenic (As) increases in agricultural soils because of anthropogenic activities and may have phytotoxic effects depending on the available concentrations. Plant performance can be improved by arbuscular mycorrhiza (AM) association under challenging conditions, such as those caused by excessive soil As levels. In this study, the influence of AM on CO2 assimilation, chlorophyll a fluorescence, SPAD-chlorophyll contents and plant growth was investigated in rice plants exposed to arsenate (AsV) or arsenite (AsIII) and inoculated or not with Rhizophagus irregularis. Under AsV and AsIII exposure, AM rice plants had greater biomass accumulation and relative chlorophyll content, increased water-use efficiency, higher carbon assimilation rate and higher stomatal conductance and transpiration rates than non-AM rice plants did. Chlorophyll a fluorescence analysis revealed significant differences in the response of AM-associated and -non-associated plants to As. Mycorrhization increased the maximum and actual quantum yields of photosystem II and the electron transport rate, maintaining higher values even under As exposure. Apart from the negative effects of AsV and AsIII on the photosynthetic rates and PSII efficiency in rice leaves, taken together, these results indicate that AM is able to sustain higher rice photosynthesis efficiency even under elevated As concentrations, especially when As is present as AsV.

Topics: Arsenates; Arsenites; Biomass; Chlorophyll; Chlorophyll A; Electron Transport; Glomeromycota; Mycorrhizae; Oryza; Photosynthesis; Photosystem II Protein Complex; Plant Leaves; Soil Pollutants; Stress, Physiological

Blue green algae Anabaena sp. was cultivated in synthetic arsenite solution to investigate its bio-oxidation potential for arsenic species. Response surface methodology (RSM) was employed based on a 3-level full factorial design considering four factors, viz. initial arsenic (III) concentration, algal dose, temperature and time. Bio-oxidation (%) of arsenic (III) was considered as response for the design. The study revealed that about 100% conversion of As (III) to As (V) was obtained for initial As (III) concentration of 2.5-7.5 mg/L at 30 °C for 72 h of exposure using 3 g/L of algal dose signifying a unique bio-oxidation potential of Anabaena sp. The dissolved CO2 (DCO2) and oxygen (DO) concentration in solution was monitored during the process and based on the data, a probable mechanism was proposed wherein algal cell acts like a catalytic membrane surface and expedites the bio-oxidation process. Bioaccumulation of arsenic, as well as, surface adsorption on algal cell was found considerably low. Lipid content of algal biomass grown in arsenite solution was found slightly lower than that of algae grown in synthetic media. Toxicity effects on algal cells due to arsenic exposure were evaluated in terms of comet assay and chlorophyll a content which indicated DNA damage to some extent along with very little decrease in chlorophyll a content. In summary, the present study explored the potential application of Anabaena sp. as an ecofriendly and sustainable option for detoxification of arsenic contaminated natural water with value-added product generation.

Topics: Adsorption; Anabaena; Arsenates; Arsenic; Arsenites; Chlorophyll; Chlorophyll A; Environmental Pollutants; Oxidation-Reduction; Solutions