phenylmercuric-acetate and leupeptin

Novel gelatinolytic activities in both latent and active forms were detected in the normal organs of rat by gelatin zymography. Multiple active bands were detected in the extracts from the skin, jejunum, muscle, and kidney without any activation. These activities were inhibited by 1,10-phenanthroline or leupeptin, nor by E64, suggesting that these activities were derived from metallo-proteinases or serine-proteinases. Some gelatinolytic active bands were newly induced or enhanced by p-aminophenylmercuric acetate. These results suggest that matrix degrading activities due to metallo- and serine-proteinases were constitutively expressed in various rat normal organs.

Topics: Animals; Cattle; Enzyme Inhibitors; Gelatin; Leupeptins; Male; Metalloendopeptidases; Phenanthrolines; Phenylmercuric Acetate; Rats; Rats, Wistar; Serine Endopeptidases