phenylglycinamide and phenylglycine-nitrile

phenylglycinamide has been researched along with phenylglycine-nitrile* in 1 studies

Other Studies

1 other study(ies) available for phenylglycinamide and phenylglycine-nitrile

Article	Year
A two-step, one-pot enzymatic synthesis of cephalexin from D-phenylglycine nitrile. Biotechnology and bioengineering, 2002, Aug-05, Volume: 79, Issue:3 A cascade of two enzymatic transformations is employed in a one-pot synthesis of cephalexin. The nitrile hydratase (from R. rhodochrous MAWE)-catalyzed hydration of D-phenylglycine nitrile to the corresponding amide was combined with the penicillin G acylase (penicillin amidohydrolase, E.C. 3.5.1.11)-catalyzed acylation of 7-ADCA with the in situ-formed amide to afford a two-step, one-pot synthesis of cephalexin. D-Phenylglycine nitrile appeared to have a remarkable selective inhibitory effect on the penicillin G acylase, resulting in a threefold increase in the synthesis/hydrolysis (S/H) ratio. 1,5-Dihydroxynaphthalene, when added to the reaction mixture, cocrystallized with cephalexin. The resulting low cephalexin concentration prevented its chemical as well as enzymatic degradation; cephalexin was obtained at 79% yield with an S/H ratio of 7.7. Topics: Acetonitriles; Catalysis; Cephalexin; Cephalosporins; Escherichia coli; Glycine; Hydro-Lyases; Models, Chemical; Multienzyme Complexes; Penicillin Amidase; Quality Control; Rhodococcus; Sensitivity and Specificity	2002

Article

Year

A two-step, one-pot enzymatic synthesis of cephalexin from D-phenylglycine nitrile.

Biotechnology and bioengineering, 2002, Aug-05, Volume: 79, Issue:3

A cascade of two enzymatic transformations is employed in a one-pot synthesis of cephalexin. The nitrile hydratase (from R. rhodochrous MAWE)-catalyzed hydration of D-phenylglycine nitrile to the corresponding amide was combined with the penicillin G acylase (penicillin amidohydrolase, E.C. 3.5.1.11)-catalyzed acylation of 7-ADCA with the in situ-formed amide to afford a two-step, one-pot synthesis of cephalexin. D-Phenylglycine nitrile appeared to have a remarkable selective inhibitory effect on the penicillin G acylase, resulting in a threefold increase in the synthesis/hydrolysis (S/H) ratio. 1,5-Dihydroxynaphthalene, when added to the reaction mixture, cocrystallized with cephalexin. The resulting low cephalexin concentration prevented its chemical as well as enzymatic degradation; cephalexin was obtained at 79% yield with an S/H ratio of 7.7.

Topics: Acetonitriles; Catalysis; Cephalexin; Cephalosporins; Escherichia coli; Glycine; Hydro-Lyases; Models, Chemical; Multienzyme Complexes; Penicillin Amidase; Quality Control; Rhodococcus; Sensitivity and Specificity

2002