perferryl-iron and potassium-persulfate

perferryl-iron has been researched along with potassium-persulfate* in 1 studies

Other Studies

1 other study(ies) available for perferryl-iron and potassium-persulfate

ArticleYear
Perferryl complex of nitric oxide synthase: role in secondary free radical formation.
    Biochimica et biophysica acta, 2002, Jan-15, Volume: 1569, Issue:1-3

    Neuronal nitric oxide synthase (NOS I) has been shown to generate nitric oxide (NO*) and superoxide (O(2)*-)during enzymatic cycling, the ratio of each free radical is dependent upon the concentration of L-arginine. Using spin trapping and electron paramagnetic resonance (EPR) spectroscopy, we recently reported that NOS I can oxidize ethanol (EtOH) to alpha-hydroxyethyl radical (CH(3)*CHOH). We speculated that the perferryl complex of NOS, (NOS-[Fe(5+)[double bond]O](3+)) was responsible for the generation of CH(3)*CHOH. Using potassium monopersulfate (KHSO(5)) to oxidize the heme of NOS I to NOS-[Fe(5+)[double bond]O](3+), we were able to demonstrate that this perferryl complex can oxidize L-arginine to L-citrulline and NO*. Even in the absence of L-arginine, EtOH was oxidized to CH(3)*CHOH by NOS-[Fe(5+)[double bond]O](3+). Sodium cyanide (NaCN), a heme blocker, inhibited the formation of CH(3)*CHOH by NOS.

    Topics: Arginine; Binding Sites; Carbon Radioisotopes; Citrulline; Electron Spin Resonance Spectroscopy; Ethanol; Free Radicals; Heme; Humans; Iron; NADP; Nitric Oxide Synthase; Nitric Oxide Synthase Type I; Oxidation-Reduction; Potassium Compounds; Recombinant Proteins; Sodium Cyanide; Spin Trapping; Sulfates

2002