peptones and podophyllic-acid

This work optimized the novel biotransformation process of podophyllotoxin to produce podophyllic acid by Pseudomonas aeruginosa CCTCC AB93066. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/l of yeast extract and 5 g/l of peptone were favorable for podophyllic acid production (i.e. 25.3+/-3.7 mg/l), while not beneficial for the cell growth of P. aeruginosa. This indicated that the accumulation of podophyllic acid was not corresponded well to the cell growth of P. aeruginosa. 0 g/l of sucrose was beneficial for podophyllic acid production (i.e. 34.3+/-3.9 mg/l), which led to high podophyllotoxin conversion (i.e. 98.2+/-0.1%). 1g/l of NaCl was the best for podophyllic acid production (i.e. 47.6+/-4.0mg/l). Secondly, the production of podophyllic acid was significantly enhanced by fed-batch biotransformation. When each 100mg/l of podophyllotoxin was added to the biotransformation system after 4, 10 and 25 h of culture, respectively, podophyllic acid concentration reached 99.9+/-12.3mg/l, enhanced by 284% comparing to one-time addition (i.e. 26.0+/-2.1mg/l). The fundamental information obtained in this study provides a simple and efficient way to produce podophyllic acid.

Topics: Analysis of Variance; Biotransformation; Kinetics; Nitrogen; Peptones; Podophyllotoxin; Pseudomonas aeruginosa; Regression Analysis; Sodium Chloride; Sucrose; Surface Properties