peoniflorin and pachymic-acid

A simple, rapid and reliable microwave-assisted extraction (MAE) combined with ultra performance liquid chromatography tandem mass spectrometry method was developed for simultaneous determination of the seven bioactive constituents in Guizhi Fuling capsule (GFC), namely gallic acid, amygdalin, albiflorin, paeoniflorin, paeonol, cinnamic acid and pachymic acid, respectively. The operation of MAE optimised through orthogonal array design experiment was performed at 80°C for 10 min with methanol-water (70:30, v/v) as the extracting solvent. The method was validated including intra- and inter-day precision, repeatability and stability, with relative standard deviation less than 3.9%, 3.3%, 4.4% and 3.1%, respectively. All analytes showed the good linearity (r >0.999), and their average recoveries varied between 98.2% and 101.2%. The results indicated that this method was simple, effective and suitable for the quality control of GFC.

Topics: Acetophenones; Amygdalin; Bridged-Ring Compounds; Capsules; Chemical Fractionation; Chromatography, High Pressure Liquid; Cinnamates; Drugs, Chinese Herbal; Gallic Acid; Glucosides; Microwaves; Monoterpenes; Quality Control; Reproducibility of Results; Tandem Mass Spectrometry; Triterpenes