peoniflorin and naringenin

The pharmacokinetic differences of paeoniflorin, naringin, naringenin and glycyrrhetinic acid (GA) following oral administration of pure compounds, single herbs and Si-Ni-San (SNS) decoction to rats were studied. Blood samples were analyzed with a validated UPLC-MS/MS method. Student's t-test was used for the statistical comparison. The Cmax and AUC0-∞ were 1470±434 ng/mL and 4663±916 ng h/mL for paeoniflorin, 64.29±59.21 ng/mL and 311.8±131.8 ng h/mL for naringin, 244.2±138.8 ng/mL and 4761±3167 ng h/mL for naringenin, and 1183±294 ng/mL and 38 994±14 377 ng h/mL for GA after oral administration of paeoniflorin, naringin and glycyrrhizic acid. The Cmax and AUC0-∞ were 812.6±259.6 ng/mL and 2489±817 ng h/mL for paeoniflorin, 344.3±234.9 ng/mL and 1479±531 ng h/mL for naringin, 981.9±465.4 ng/mL and 12 284±6378 ng h/mL for naringenin, and 3164±742 ng/mL and 78 817±16 707 ng h/mL for GA after oral administration of SNS decoction. There were significant differences between the pharmacokinetic behavior after oral administration of SNS decoction compared with pure components or herbs. The results indicated that some components in the other herbs of SNS had a pharmacokinetic interaction with paeoniflorin, naringin, naringenin and GA.

Topics: Administration, Oral; Animals; Drugs, Chinese Herbal; Flavanones; Glucosides; Glycyrrhetinic Acid; Male; Monoterpenes; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry

A UPLC-MS/MS method was developed for the simultaneous determination of paeoniflorin, naringin, naringenin and glycyrrhetinic acid in rat plasma. A Waters BEH C(18) column was used with a gradient mobile phase system of methanol-water containing 2 mM ammonium acetate. The analysis was performed on a positive ionization electrospray mass spectrometer via multiple reaction monitoring (MRM). One-step protein precipitation with acetonitrile was used to extract the analytes from plasma. The limits of quantification were 9.800 ng/ml for paeoniflorin, 5.100 ng/ml for naringin, 5.200 ng/ml for naringenin and 10.60 ng/ml for glycyrrhetinic acid, respectively. The intra- and inter-day precision (relative standard deviation, RSD) ranged 4.9-12% and 2.8-13%, respectively. The accuracy (relative error, RE) was from -7.3% to 7.5% at all quality control (QC) levels. The validated method was applied to a pharmacokinetic study in rats after oral administration of Si-Ni-San decoction.

Topics: Administration, Oral; Animals; Benzoates; Bridged-Ring Compounds; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Flavanones; Glucosides; Glycyrrhetinic Acid; Limit of Detection; Male; Monoterpenes; Quality Control; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry