pectins and malic-acid

The aim of this study was to investigate the biochemical and metabolic changes, related to oxidative stress, ethylene and respiration, cell wall modification and primary metabolism, between a high ('Prime Giant') and a low ('Cristalina') cracking susceptible sweet cherry cultivar during growth and ripening. While cherries are referred as a non-climacteric fruit, our results show that an increase of endogenous ethylene production at earlier fruit developmental stages is parallel to colour development and softening during growth. Higher cracking susceptibility was clearly associated to a higher fruit growth rate and accompanied by an increase net CO

Topics: Adaptation, Physiological; Biomarkers; Biomass; Cell Respiration; Ethylenes; Fructose; Fruit; Glucose; Hydrogen Peroxide; Malates; Malondialdehyde; Methyltransferases; Oxidative Stress; Pectins; Polygalacturonase; Prunus avium

Nitric oxide (NO) is an important bioactive molecule involved in cell wall metabolism, which has been recognized as a major target of aluminium (Al) toxicity. We have investigated the effects of Al-induced NO production on cell wall composition and the subsequent Al-binding capacity in roots of an Al-sensitive cultivar of wheat (Triticum aestivum L. cv. Yang-5). We found that Al exposure induced NO accumulation in the root tips. Eliminating NO production with an NO scavenger (cPTIO) significantly alleviated the Al-induced inhibition of root growth and thus reduced Al accumulation. Elimination of NO, however, did not significantly affect malate efflux or rhizosphere pH changes under Al exposure. Levels of cell wall polysaccharides (pectin, hemicelluloses 1, and hemicelluloses 2) and pectin methylesterase activity, as well as pectin demethylation in the root apex, significantly increased under Al treatment. Exogenous cPTIO application significantly decreased pectin methylesterase activity and increased the degree of methylation of pectin in the root cell wall, thus decreasing the Al-binding capacity of pectin. These results suggest that the Al-induced enhanced production of NO decreases cell wall pectin methylation, thus increasing the Al-binding capacity of pectin and negatively regulating Al tolerance in wheat.

Topics: Aluminum; Benzoates; Carboxylic Ester Hydrolases; Cell Wall; Evans Blue; Free Radical Scavengers; Hydrogen-Ion Concentration; Imidazoles; Malates; Methylation; Models, Biological; Nitric Oxide; Pectins; Plant Roots; Triticum; Uronic Acids

Pectic acids participate in the transport of heavy metal ions in the root apoplasm by establishing interactions that can lead to their partial or total immobilization. The ions accumulated can be mobilized by phenolic compounds and organic acids of the root exudates. In this context, we tested, in aqueous phase, the ability of malic acid and esculetine (ESC) to mobilize the Cu(II) ions accumulated in a Ca-polygalacturonate matrix (Ca-PGA) used as a model of the root apoplasm. The results show that at pH 5.0 and 6.0 malic acid mobilizes about 22% and 34% of the Cu(II) accumulated, respectively, whereas ESC about 12% and 25%. ESC was found to cause the reduction of Cu(II) to Cu(I) with formation of ESC oxidation products. The study of the Cu(II)-ESC binary system evidenced that one molecule of ESC reduces one Cu(II) ion with formation of semiquinonic radicals that couple to form two dimers. The Cu(II) reduction by ESC was found faster in the presence of malic acid.

Topics: Biopolymers; Calcium; Copper; Malates; Molecular Structure; Oxidation-Reduction; Pectins

With the aim to investigate the role of the polyuronic components in the accumulation of iron and phosphate at the soil-root interface, the interactions of Ca-polygalacturonates (PGAs) with Fe(III) and P ions and of Fe(III)-Ca-polygalacturonates (Fe-PGAs) with P ions were studied at pH 4.7. The role of citric, malic and pyruvic acids in the mobilization of Fe(III) and P, in the presence and absence of Ca(II) 2.5mM, was also investigated. The sorption kinetics evidenced that P diffuses freely through the calcium polysaccharidic matrix whereas Fe(III) accumulates as an hydroxypolymer. The sorption kinetics of P by the Fe-PGA indicated that the amount of P sorbed increases with increasing its initial concentration up to a constant value equal to 0.98micromol/3.87micromolmg(-1) of Fe(III)-polymer trapped. The FT-IR spectra of the P-Fe-PGA systems, show bands attributable to P-O(H) stretching vibrations. The study of systems with a constant initial P amount and varying Fe(III) amounts allowed to hypothesize that phosphate settles down inside holes formed by the carboxylate groups of galacturonic units. Citric and malic acids showed to be active in the mobilization of both Fe and P whereas pyruvic acid appeared inactive.

Topics: Carbohydrate Conformation; Citric Acid; Iron; Kinetics; Malates; Pectins; Phosphates; Plant Roots; Pyruvic Acid; Soil; Time Factors; Uronic Acids