pectins and hesperetin

Different ratios of hesperetin (HT) were successfully grafted onto pectin from basic water (PB) molecules via free radical-induced reaction. The structure of PB-HT conjugates was characterized by ultraviolet spectroscopy, infrared spectroscopy, X-ray diffraction and scanning electron microscopy. Results indicated that HT was successfully grafted onto pectin molecules, and PB-HT-0.5 showed the highest HT content (103.18 ± 2.76 mg/g). Thermogravimetric analysis indicated that HT crystals showed good thermal resistance and could improve the thermal stability of PB-HT conjugates. Additionally, PB-HT conjugates showed good cytocompatibility and blood compatibility. This study provides a novel and efficient method to synthesize hesperetin-grafted pectin conjugate, which showed potential application in the fields of functional foods in the future.

Topics: Hesperidin; Pectins; X-Ray Diffraction

Orange pomace polyphenols have potential for use as nutraceutical ingredients in functional foods and beverages. However, owing to their low water solubility and bioaccessibility, they are not being utilized to their full potential. The goal of this research is to assess the impact of encapsulation on hesperetin (HT - a model orange polyphenol) water solubility, antioxidant activity, and in vitro bioaccessibility.. In this study, a citrus flavonoid aglycone, HT, was encapsulated within water-dispersible colloidal complexes (d = 350 ± 8 nm) formed by electrostatic attraction of pea protein isolate and high-methoxyl pectin at a mixing ratio of 1:1 (v/v) and pH 4. The maximum amount of HT that could be dispersed in water was much higher for the encapsulated form (99 ± 7 μg mL. These results suggest that a well-designed, biopolymer-based delivery system may improve the effective incorporation of HT, and potentially other orange pomace polyphenols, into food and beverage products. This could provide an additional high-value use for orange juicing by-products while introducing a new nutraceutical product to the food and beverage industry. © 2022 Society of Chemical Industry.

Topics: Citrus; Citrus sinensis; Flavonoids; Hesperidin; Pea Proteins; Pectins; Polyphenols; Static Electricity; Water

In this study three feed additives (hesperetin, naringenin and pectin) for laying hens were investigated on their influence on the egg yolk cholesterol, serum traits and antioxidant activities in hens. Additives were extracted from citrus and grapefruit peels and contained 31.5% crude hesperetin, 39% crude naringenin and 60% galacturonic acid (pectin). Eighty 30-week-old Leghorn laying hens were randomly assigned to four groups and received, for two months, a control diet or diets with 0.05% hesperetin, 0.05% naringenin or 0.5% pectin. All additives reduced the egg yolk cholesterol level significantly. Feeding diets with added flavonoids (hesperetin and naringenin) increased the yolk weight and the ratio of yolk weight/egg weight and the blood serum superoxide dismutase (SOD) activity was elevated. Total antioxidation capacity, the level of thiobarbituric acid-reactive substances and superoxide scavenging capacity in the naringenin group were greater than in the control group. Supplemented flavonoids reduced the serum cholesterol level significantly, while serum triglyceride concentration in the naringenin and pectin groups was reduced. Addition of flavonoids resulted in an enhanced cholesterol level in excreta. The results of this study indicated that intake of hesperetin, naringenin and pectin extracted from citrus and grapefruit peel in laying hens diet, may exhibit positive effects.

Topics: Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Antioxidants; Chickens; Cholesterol; Egg Yolk; Eggs; Female; Flavanones; Free Radical Scavengers; Hesperidin; Lipid Metabolism; Pectins; Random Allocation; Superoxide Dismutase; Thiobarbituric Acid Reactive Substances; Triglycerides