pectins has been researched along with 8-amino-1-3-6-naphthalenetrisulfonic-acid* in 2 studies
2 other study(ies) available for pectins and 8-amino-1-3-6-naphthalenetrisulfonic-acid
| Article | Year |
|---|---|
Capillary electrophoresis with detection by laser-induced fluorescence.
The importance of capillary zone electrophoresis (CZE) has been increasing in use for: structural analysis of plant cell walls, characterization of enzymes that degrade polysaccharides, and profiling of oligosaccharides to characterize cell wall mutants. CZE with laser-induced fluorescence detection provides high separation efficiencies, high speed analysis, with extremely small sample requirements. Here, we describe the instrumentation we use and the methods for attaching fluorescent labels to oligosaccharides so that they can be detected. Topics: Cotyledon; Electrophoresis, Capillary; Enzyme Assays; Fluorescence; Fluorescent Dyes; Gossypium; Lasers; Naphthalenes; Pectins; Polysaccharide-Lyases; Pyrenes | 2011 |
Detection and differentiation of pectic enzyme activity in vitro and in vivo by capillary electrophoresis of products from fluorescent-labeled substrate.
A sensitive assay is described for the detection of pectate-depolymerizing enzymes using capillary electrophoresis of a fluorescent end-labeled pectate oligomer. The labeled oligomer is allowed to react with the enzyme either in vitro or in vivo, such as inside the intercellular spaces of a cotton cotyledon, and after an appropriate incubation time the products are analyzed by capillary electrophoresis. The site and mode of action of the pectate-depolymerizing activity can be inferred from the products. Both endo- and exopolygalacturonase activity, and lyase activity, were distinguished. Since only the fluorescent oligomer and products from its labeled reducing end are detected, there is no interference from other compounds; only pectic enzyme activity is detected. By this type of analysis we can show that there is considerable endo- and exo-polygalacturonase activity in the intercellular spaces of cotton cotyledons. Topics: Cotyledon; Electrophoresis, Capillary; Fluorescence; Glycoside Hydrolases; Gossypium; Hexuronic Acids; Lyases; Naphthalenes; Pectins; Polygalacturonase; Sensitivity and Specificity; Substrate Specificity | 1996 |