pectins and (3-chloro-2-hydroxypropyl)trimethylammonium-chloride

pectins has been researched along with (3-chloro-2-hydroxypropyl)trimethylammonium-chloride* in 1 studies

Other Studies

1 other study(ies) available for pectins and (3-chloro-2-hydroxypropyl)trimethylammonium-chloride

Article	Year
Synthesis, characterization, and self-assembly with plasmid DNA of a quaternary ammonium derivative of pectic galactan and its fluorescent labeling for bioimaging applications. Carbohydrate polymers, 2016, Oct-05, Volume: 150 Quaternized derivatives of pectic galactan (QPG) were synthesized by a reaction of pectic galactan (PG) with 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (CHPTAC) in the presence of aqueous sodium hydroxide solution under mild reaction conditions. The results showed that the concentration of CHPTAC and NaOH has great impact on the quaternization reaction. QPG was found to interact electrostatically with plasmid DNA in aqueous solution to form complexes in globular condensed morphology in a nanometer scale size ranging from 60 to 160nm. Complexes formed with QPG fluorescently labeled with 5-DTAF (QPG-5-DTAF) were introduced to the C6 rat glioma cell line, and were found to be able to enter the cell and approach the nucleus within 24h. The results suggest that this type of modified natural polysaccharide may have an advantage as a biocompatible and biodegradable gene delivery carrier and furthermore may serve as a cell specific carrier. Topics: Animals; Cell Line, Tumor; Chemistry Techniques, Synthetic; DNA; Drug Carriers; Fluoresceins; Fluorescent Dyes; Galactans; Molecular Imaging; Pectins; Plasmids; Propanols; Quaternary Ammonium Compounds; Rats; Sodium Hydroxide; Transfection; Water	2016

Article

Year

Synthesis, characterization, and self-assembly with plasmid DNA of a quaternary ammonium derivative of pectic galactan and its fluorescent labeling for bioimaging applications.

Carbohydrate polymers, 2016, Oct-05, Volume: 150

Quaternized derivatives of pectic galactan (QPG) were synthesized by a reaction of pectic galactan (PG) with 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (CHPTAC) in the presence of aqueous sodium hydroxide solution under mild reaction conditions. The results showed that the concentration of CHPTAC and NaOH has great impact on the quaternization reaction. QPG was found to interact electrostatically with plasmid DNA in aqueous solution to form complexes in globular condensed morphology in a nanometer scale size ranging from 60 to 160nm. Complexes formed with QPG fluorescently labeled with 5-DTAF (QPG-5-DTAF) were introduced to the C6 rat glioma cell line, and were found to be able to enter the cell and approach the nucleus within 24h. The results suggest that this type of modified natural polysaccharide may have an advantage as a biocompatible and biodegradable gene delivery carrier and furthermore may serve as a cell specific carrier.

Topics: Animals; Cell Line, Tumor; Chemistry Techniques, Synthetic; DNA; Drug Carriers; Fluoresceins; Fluorescent Dyes; Galactans; Molecular Imaging; Pectins; Plasmids; Propanols; Quaternary Ammonium Compounds; Rats; Sodium Hydroxide; Transfection; Water

2016