pactamycin and 3-aminobenzoic-acid

pactamycin has been researched along with 3-aminobenzoic-acid* in 4 studies

Other Studies

4 other study(ies) available for pactamycin and 3-aminobenzoic-acid

ArticleYear
Functional Characterization of 3-Aminobenzoic Acid Adenylation Enzyme PctU and UDP-N-Acetyl-d-Glucosamine: 3-Aminobenzoyl-ACP Glycosyltransferase PctL in Pactamycin Biosynthesis.
    Chembiochem : a European journal of chemical biology, 2019, 10-01, Volume: 20, Issue:19

    Pactamycin is an antibiotic produced by Streptomyces pactum with antitumor and antimalarial properties. Pactamycin has a unique aminocyclitol core that is decorated with 3-aminoacetophenone, 6-methylsaliciate, and an N,N-dimethylcarbamoyl group. Herein, we show that the adenylation enzyme PctU activates 3-aminobenzoic acid (3ABA) with adenosine triphosphate and ligates it to the holo form of the discrete acyl carrier protein PctK to yield 3ABA-PctK. Then, 3ABA-PctK is N-glycosylated with uridine diphosphate-N-acetyl-d-glucosamine (UDP-GlcNAc) by the glycosyltransferase PctL to yield GlcNAc-3ABA-PctK. Because 3ABA is known to be a precursor of the 3-aminoacetophenone moiety, PctU appears to be a gatekeeper that selects the appropriate 3-aminobenzoate starter unit. Overall, we propose that acyl carrier protein-bound glycosylated 3ABA derivatives are biosynthetic intermediates of pactamycin biosynthesis.

    Topics: Adenine; Adenylate Kinase; Bacterial Proteins; Enzymes; Glycosyltransferases; meta-Aminobenzoates; Pactamycin; Uridine Diphosphate N-Acetylglucosamine

2019
Mechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.
    Chembiochem : a European journal of chemical biology, 2015, Volume: 16, Issue:17

    Mutational analysis of the pyridoxal 5'-phosphate (PLP)-dependent enzyme PctV was carried out to elucidate the multi-step reaction mechanism for the formation of 3-aminobenzoate (3-ABA) from 3-dehydroshikimate (3-DSA). Introduction of mutation K276R led to the accumulation of a quinonoid intermediate with an absorption maximum at 580 nm after the reaction of pyridoxamine 5'-phosphate (PMP) with 3-DSA. The chemical structure of this intermediate was supported by X-ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3-ABA. They also indicate that Lys276 (in the active site of PctV) plays multiple roles, including acid/base catalysis during the dehydration reaction of the quinonoid intermediate.

    Topics: Binding Sites; Biocatalysis; Catalytic Domain; Crystallography, X-Ray; Kinetics; meta-Aminobenzoates; Molecular Dynamics Simulation; Mutagenesis, Site-Directed; Oxidoreductases; Pactamycin; Pyridoxal Phosphate; Shikimic Acid; Spectrophotometry, Ultraviolet

2015
Mutasynthesis of fluorinated pactamycin analogues and their antimalarial activity.
    Organic letters, 2013, Apr-05, Volume: 15, Issue:7

    A mutasynthetic strategy has been used to generate fluorinated TM-025 and TM-026, two biosynthetically engineered pactamycin analogues produced by Streptomyces pactum ATCC 27456. The fluorinated compounds maintain excellent activity and selectivity toward chloroquine-sensitive and multidrug-resistant strains of malarial parasites as the parent compounds. The results also provide insights into the biosynthesis of 3-aminobenzoic acid in S. pactum.

    Topics: Antimalarials; Chloroquine; Drug Resistance; Drug Resistance, Multiple; Hydrocarbons, Fluorinated; meta-Aminobenzoates; Molecular Structure; Pactamycin; Plasmodium falciparum; Streptomyces

2013
A single PLP-dependent enzyme PctV catalyzes the transformation of 3-dehydroshikimate into 3-aminobenzoate in the biosynthesis of pactamycin.
    Chembiochem : a European journal of chemical biology, 2013, Jul-08, Volume: 14, Issue:10

    Natural amino donation: A PLP-dependent aminotransferase PctV, encoded in the pactamycin biosynthetic gene cluster, was found to catalyze the formation of 3-aminobenzoate from 3-dehydroshikimate with L-glutamate as the amino donor. The PctV reaction comprises a transamination and two dehydration reactions. This is the first report of a simple 3-ABA synthase in nature.

    Topics: Biological Products; Catalysis; Magnetic Resonance Spectroscopy; meta-Aminobenzoates; Models, Molecular; Pactamycin; Shikimic Acid; Streptomyces; Substrate Specificity

2013