oxypaeoniflora and paeonol

Topics: Acetophenones; Animals; Anthocyanins; Anti-Inflammatory Agents; Benzoates; Bridged-Ring Compounds; Cardiovascular Agents; Drugs, Chinese Herbal; Glucosides; Medicine, Chinese Traditional; Medicine, East Asian Traditional; Monoterpenes; Plant Extracts; Plants, Medicinal; Terpenes

In this study, a improved high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous quantification of twelve major components belonging to three chemical types was developed and validated, and was applied to quantitatively compare the quality of Radix Paeoniae Alba (RPA) sulfur-fumigated with different durations and purchased from commercial herbal markets. The contents of paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoic acid and paeonol decreased whereas that of paeoniflorin sulfonate increased in RPA with the extending of sulfur-fumigation duration. Different levels of paeoniflorin sulfonate were determined in ten of seventeen commercial RPA samples, indicating that these ten samples may be sulfur-fumigated with different durations. Moreover, the relative standard deviation of the contents of each component was higher in the commercial sulfur-fumigated RPA samples than that in commercial non-fumigated RPA samples, and the percentage of the total average content of monoterpene glycosides in the determined analytes was higher in the decoctions of commercial sulfur-fumigated RPA than that in commercial non-fumigated RPA samples. All these results suggested that the established method was precise, accurate and sensitive enough for the global quality evaluation of sulfur-fumigated RPA, and sulfur-fumigation can not only change the proportions of bioactive components, but also cause the reduction of the quality consistency of both raw materials and aqueous decoctions of RPA.

Topics: Acetophenones; Benzoic Acid; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Fumigation; Glucosides; Glycosides; Herbal Medicine; Monoterpenes; Paeonia; Plant Roots; Sulfur

To analyze the chemical constituents of cortex moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.. An on-line optimized HPLC-DAD/MS2 technique was employed.. In the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated. Among them, over thirty compounds were identified, including paeonol, paeonilflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, galloyloxypaeoniflorin, mundanpioside A, C, D, E, H, etc. by the high performance liquid chromatography with diode-array detection in parallel with electrospray ionization and quadrupole-time of flight tandem mass spectrometry (HPLC-DAD/ESI-MS2).. This method can be used to rapidly determine the constituents of cortex moutan.

Topics: Acetophenones; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Glucosides; Molecular Structure; Monoterpenes; Paeonia; Plant Extracts; Plants, Medicinal; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry

To establish a HPLC fingerprint for quality evaluation of Cortex Moutan.. The HPLC chromatographic fingerprinting of 30 lots of Cortex Moutan was established and major peaks were identified by LC-MS and MS-MS.. The HPLC fingerprint of Cortex Moutan was established, showing 15 characteristic peaks. The areas of these peaks were found to complied with the following rule: paeonol > 1, 2, 3, 4, 6-penta-O-galloyl-glucos > methyl gallate > galloylpaeoniflorin > gallic acid > oxypaeoniflorin > other compounds.. The chromatographic fingerprinting of Cortex Moutan with high specificity can be used to control its quality and monitor lot to lot consistency.

Topics: Acetophenones; Bridged Bicyclo Compounds, Heterocyclic; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Gallic Acid; Glucosides; Hydrolyzable Tannins; Monoterpenes; Paeonia; Plant Bark; Plants, Medicinal; Quality Control

Capillary electrophoresis with electrochemical detection has been employed for the separation and determination of the three active constituents (paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin) in traditional Chinese medicine, Moutan Cortex (root cortex of Paeonia suffruticosa Andrews). The effects of several important factors, such as the concentration of running buffer, the separation voltage, the injection time, and the detection potential, were investigated to determine the optimum conditions. The detection electrode was a 300 microm diameter carbon-disc electrode at a working potential of +0.90 V (versus SCE). The three analytes could be well separated within 7 min in a 40 cm length fused-silica capillary at a separation voltage of 12 kV in a 50 mM borate buffer (pH 9.2). The relation between the peak current and the analyte concentration was linear over 3 orders of magnitude with detection limits (S/N = 3) ranging from 0.4 to 0.7 microM for all analytes. The proposed method has been successfully applied to the determination of paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin in real plant samples with satisfactory assay results.

Topics: Acetophenones; Drugs, Chinese Herbal; Electrochemistry; Electrophoresis, Capillary; Glycosides; Paeonia; Terpenes