oxypaeoniflora and benzoylpaeoniflorin

Topics: Acetophenones; Animals; Anthocyanins; Anti-Inflammatory Agents; Benzoates; Bridged-Ring Compounds; Cardiovascular Agents; Drugs, Chinese Herbal; Glucosides; Medicine, Chinese Traditional; Medicine, East Asian Traditional; Monoterpenes; Plant Extracts; Plants, Medicinal; Terpenes

We aimed to compare the estrogenic activities of compounds isolated from Moutan Cortex Radicis (MRC, Paeonia suffruticosa Andrews) and identify their potential use in hormone replacement therapy. We quantified seven marker components (gallic acid, oxypaeoniflorin, paeoniflorin, ethyl gallate, benzoic acid, benzoylpaeoniflorin, and paeonol) in MRC using a high-performance liquid chromatography simultaneous analysis assay. To investigate the estrogenic activity of MRC and the seven marker components, an E-screen assay was conducted using the estrogen receptor (ER)-positive MCF-7 human breast cancer cell line. Among them, ethyl gallate caused cell proliferation in a concentration-dependent manner at concentrations above 25 µM and was clearly suppressed by combination treatment with the ER antagonist ICI 182,780. Therefore, ethyl gallate may be a compound of MRC that can increase the estrogenic effect in ER-positive MCF-7 cells.

Topics: Cell Line, Tumor; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Estrogens; Estrone; Gallic Acid; Glucosides; Hormone Replacement Therapy; Humans; Monoterpenes; Paeonia; Protein Binding; Structure-Activity Relationship

Topics: Administration, Intravenous; Animals; Chalcone; Chromatography, High Pressure Liquid; Coumaric Acids; Drugs, Chinese Herbal; Glucosides; Limit of Detection; Linear Models; Male; Monoterpenes; Quinones; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Tandem Mass Spectrometry

In this study, a improved high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous quantification of twelve major components belonging to three chemical types was developed and validated, and was applied to quantitatively compare the quality of Radix Paeoniae Alba (RPA) sulfur-fumigated with different durations and purchased from commercial herbal markets. The contents of paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoic acid and paeonol decreased whereas that of paeoniflorin sulfonate increased in RPA with the extending of sulfur-fumigation duration. Different levels of paeoniflorin sulfonate were determined in ten of seventeen commercial RPA samples, indicating that these ten samples may be sulfur-fumigated with different durations. Moreover, the relative standard deviation of the contents of each component was higher in the commercial sulfur-fumigated RPA samples than that in commercial non-fumigated RPA samples, and the percentage of the total average content of monoterpene glycosides in the determined analytes was higher in the decoctions of commercial sulfur-fumigated RPA than that in commercial non-fumigated RPA samples. All these results suggested that the established method was precise, accurate and sensitive enough for the global quality evaluation of sulfur-fumigated RPA, and sulfur-fumigation can not only change the proportions of bioactive components, but also cause the reduction of the quality consistency of both raw materials and aqueous decoctions of RPA.

Topics: Acetophenones; Benzoic Acid; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Fumigation; Glucosides; Glycosides; Herbal Medicine; Monoterpenes; Paeonia; Plant Roots; Sulfur