oxalylglycine and thiazolyl-blue

Hypoxia mimetic agents (HMAs) have been shown to have a positive influence on cellular functions in a multitude of tissue regenerative strategies. Novel experimental approaches use biomaterials as carriers for controlled delivery of these HMAs. Here, the cytotoxic aspects of biocompatibility are of key relevance. The MTT assay is widely used to evaluate cytotoxicity and proliferation. Based on the implications from the proceeding research we hypothesized that specific HMAs such as deferoxamine at high concentrations can interfere with the MTT assay. Thus, the aim of this study was to test the repercussions of the HMAs dimethyloxalylglycine, deferoxamine, L-mimosine, and CoCl

Topics: 3T3 Cells; Amino Acids, Dicarboxylic; Animals; Biocompatible Materials; Cell Line; Cell Survival; Cobalt; Deferoxamine; Dimethyl Sulfoxide; Mice; Mimosine; Tetrazolium Salts; Thiazoles