ovalbumin and hypusine

ovalbumin has been researched along with hypusine* in 1 studies

Other Studies

1 other study(ies) available for ovalbumin and hypusine

ArticleYear
Synthesis of reagents for the construction of hypusine and deoxyhypusine peptides and their application as peptidic antigens.
    Journal of medicinal chemistry, 1998, Sep-24, Volume: 41, Issue:20

    Two new synthetic methods which allow access to (2S)-deoxyhypusine, natural (2S,9R)-hypusine, (2S,9S)-hypusine, and deoxyhypusine- and hypusine-containing peptides are described. The methods involve both the construction of a deoxyhypusine reagent in which the alpha-nitrogen protecting group is orthogonal to the N-7 and N-12 protecting groups and an alternate synthesis of our previous hypusine reagent, a synthesis which provides for better stereochemical control at C-9. Synthetic hypusine and deoxyhypusine can be generated from these reagents. The hypusine-containing hexapeptide (Cys-Thr-Gly-Hpu-His-Gly) is conjugated to ovalbumin (OVA), keyhole limpet hemocyanin (KLH), and a bis-maleimide; KLH conjugates are also made with the deoxyhypusine- and lysine-containing hexapeptides. Monoclonal antibodies are generated to the hypusine-containing hexapeptide-OVA conjugate in mice. These are isolated and screened against the hypusine-containing hexapeptide-KLH and hypusine-containing hexapeptide-bis-maleimide conjugates, as well as against the deoxyhypusine-containing and lysine-containing hexapeptide-KLH conjugates. These antibodies may be useful in localizing intracellular hypusine-containing peptides as well as peptides containing hypusine analogues.

    Topics: Animals; Antibodies, Monoclonal; CHO Cells; Cricetinae; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Eukaryotic Translation Initiation Factor 5A; Haptens; Hemocyanins; Humans; Lysine; Maleimides; Mice; Mice, Inbred BALB C; Oligopeptides; Ovalbumin; Peptide Initiation Factors; RNA-Binding Proteins; Stereoisomerism

1998
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