ovalbumin and hypusine
ovalbumin has been researched along with hypusine* in 1 studies
Other Studies
1 other study(ies) available for ovalbumin and hypusine
Article | Year |
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Synthesis of reagents for the construction of hypusine and deoxyhypusine peptides and their application as peptidic antigens.
Two new synthetic methods which allow access to (2S)-deoxyhypusine, natural (2S,9R)-hypusine, (2S,9S)-hypusine, and deoxyhypusine- and hypusine-containing peptides are described. The methods involve both the construction of a deoxyhypusine reagent in which the alpha-nitrogen protecting group is orthogonal to the N-7 and N-12 protecting groups and an alternate synthesis of our previous hypusine reagent, a synthesis which provides for better stereochemical control at C-9. Synthetic hypusine and deoxyhypusine can be generated from these reagents. The hypusine-containing hexapeptide (Cys-Thr-Gly-Hpu-His-Gly) is conjugated to ovalbumin (OVA), keyhole limpet hemocyanin (KLH), and a bis-maleimide; KLH conjugates are also made with the deoxyhypusine- and lysine-containing hexapeptides. Monoclonal antibodies are generated to the hypusine-containing hexapeptide-OVA conjugate in mice. These are isolated and screened against the hypusine-containing hexapeptide-KLH and hypusine-containing hexapeptide-bis-maleimide conjugates, as well as against the deoxyhypusine-containing and lysine-containing hexapeptide-KLH conjugates. These antibodies may be useful in localizing intracellular hypusine-containing peptides as well as peptides containing hypusine analogues. Topics: Animals; Antibodies, Monoclonal; CHO Cells; Cricetinae; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Eukaryotic Translation Initiation Factor 5A; Haptens; Hemocyanins; Humans; Lysine; Maleimides; Mice; Mice, Inbred BALB C; Oligopeptides; Ovalbumin; Peptide Initiation Factors; RNA-Binding Proteins; Stereoisomerism | 1998 |