ovalbumin and ethyl-acetate

A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization.

Topics: Acetates; Adjuvants, Immunologic; Animals; Antibodies; Antigens; Contraception, Immunologic; Escherichia coli; Female; Freeze Drying; Gonadotropin-Releasing Hormone; Immunization; Methylene Chloride; Mycobacterium; Oligodeoxyribonucleotides; Organ Size; Ovalbumin; Ovary; Rats; Rats, Sprague-Dawley; Solvents; Vaccines

Argemone platyceras is used in Mexico as a remedy for cough, bronchitis and pneumonia. The present study was performed to investigate the pharmacological anti-asthmatic properties of Argemone platyceras on airways and to identify its active principles. Methanol extracts of leaves and flowers, subsequent organic and aqueous extraction phases, and silica gel chromatography fractions were assayed on the carbachol-induced response, and/or on ovalbumin antigenic challenge, and on leukotriene D(4)-induced response of tracheae from sensitized and non-sensitized guinea-pigs. Methanol extracts, ethyl-acetate phase, and its fractions 6 and 7 inhibited the carbachol-induced contractile response. Isoquercitrin and rutin were the main compounds found in fractions 6 and 7 respectively. Isoquercitrin (fraction 6) abolished the response to ovalbumin, and decreased the contractile response to leukotriene D(4). Because of its effect on carbachol-induced contractile response, on the late-phase response to ovalbumin, and on leukotriene D(4)-induced contractile response, isoquercitrin might be highly useful in treatment of asthma.

Topics: Acetates; Animals; Anti-Asthmatic Agents; Anti-Inflammatory Agents, Non-Steroidal; Argemone; Asthma; Bronchoconstriction; Carbachol; Chemical Fractionation; Dose-Response Relationship, Drug; Flowers; Guinea Pigs; Indomethacin; Leukotriene D4; Magnetic Resonance Spectroscopy; Male; Methanol; Molecular Structure; Muscle Contraction; Ovalbumin; Phytotherapy; Plant Leaves; Quercetin; Rutin; Time Factors; Trachea