ovalbumin and dehydrocostus-lactone

We previously reported that the biologically active form of histamine releasing factor (HRF) is dimerized translationally controlled tumor protein (dTCTP) which is involved in a number of allergic diseases.. Hoping that agents that modulate dTCTP may provide new therapeutic targets to allergic inflammatory diseases, we screened a library of natural products for substances that inhibit dTCTP. One such inhibitor we found was dehydrocostus lactone (DCL), a natural sesquiterpene present in rhizome of Saussurea lappa Clarke, the subject of this study.. We evaluated the therapeutic efficacy of DCL in a mouse model of ovalbumin (OVA)-induced allergic airway inflammation, employing the ELISA system using BEAS-2B cells and splenocytes, and confirmed that DCL interacts with dTCTP using SPR assay.. DCL inhibited dTCTP-induced secretion of IL-8 in BEAS-2B cells. From kinetic analysis of dTCTP and DCL, we found that K. DCL's therapeutic potential in allergic airway inflammation is based on its anti-inflammatory activity of suppressing the function of dTCTP.

Topics: Animals; Asthma; Biomarkers, Tumor; Bronchoalveolar Lavage Fluid; Cytokines; Disease Models, Animal; Female; Hypersensitivity; Immunoglobulin E; Inflammation; Kinetics; Lactones; Mice, Inbred BALB C; Ovalbumin; Protein Multimerization; Saussurea; Sesquiterpenes; Surface Plasmon Resonance; Tumor Protein, Translationally-Controlled 1