ovalbumin and daphnetin

Allergic rhinitis (AR) is an inflammatory disorder of nasal mucosa resulting from allergen exposure. Daphnetin (DAP) is a coumarin derivative that has various bioactivities. Nevertheless, its specific function in AR is unclear.. This study is aimed to explore the specific function of DAP in AR.. An AR murine model was established by ovalbumin (OVA) induction. Murine sneezing and rubbing behaviors were observed. Hematoxylin-eosin was used for histopathological observation of nasal mucosa. ELISA was utilized for detection of cytokine production in murine serum. Oxidative stress-associated markers were assessed by commercial assay kits. Western blotting was utilized for evaluating protein levels of Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) in nasal mucosa.. DAP alleviated OVA-induced nasal symptoms, inflammatory response and oxidative stress in the AR murine model. DAP activated nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) signaling and inactivated TLR4/NF-κB signaling in murine nasal mucosa.. DAP mitigates OVA-induced AR in mice by activating Nrf2/HO-1 signaling and inactivating TLR4/NF-κB signaling.

Topics: Animals; Disease Models, Animal; Mice; Mice, Inbred BALB C; Nasal Mucosa; NF-E2-Related Factor 2; NF-kappa B; Ovalbumin; Rhinitis, Allergic; Toll-Like Receptor 4

Sixty BALB/c mice were divided into six groups. The emulsion (100 μL containing 100 μg OVA) was injected subcutaneously with OVA + CFA into the shaved backs of the BALB/c mice on day 1, and a boosting injection was administered in OVA + IFA 2 weeks later. Beginning on the day of immunization, the immunized mice were administered intraperitoneally with daphnetin at a dose of 5, 10, and 20 mg/kg in saline solution for 28 consecutive days. We measured the effect of daphnetin on OVA-specific antibody, cytokine production, and Splenocyte proliferation. The results revealed that daphnetin significantly suppressed serum immunoglobulin G levels (IgG), and the OVA-specific IgG subclasses IgG1 and IgG2b, daphnetin was also significantly decreased the Th1 and Th2 cytokine productions, inhibited the splenocytes proliferation rate. It proved that daphnetin could suppress humoral response activity on OVA-sensitized mice, suggesting a potential role on daphnetin as a new immunosuppressive drug.

Topics: Animals; Cells, Cultured; Dose-Response Relationship, Drug; Immunity, Humoral; Immunosuppressive Agents; Injections, Subcutaneous; Mice; Mice, Inbred BALB C; Ovalbumin; Spleen; Umbelliferones