ovalbumin and calconcarboxylic-acid

ovalbumin has been researched along with calconcarboxylic-acid* in 1 studies

Other Studies

1 other study(ies) available for ovalbumin and calconcarboxylic-acid

Article	Year
Mixed-dye staining method for protein detection in polyacrylamide gel electrophoresis using calconcarboxylic acid and rhodamine B. Electrophoresis, 1998, Volume: 19, Issue:14 We have developed a new mixed-dye protein staining method that is simple, rapid, and sensitive. A freshly prepared mixture of calconcarboxylic acid (NN, 0.02%) and rhodamine B (RB, 0.04%) in 40% methanol/7% acetic acid, was used as a staining solution. RB acts as an auxiliary agent to inhibit the binding of NN to the gel matrix, reducing the background staining and therefore enhancing the protein staining by NN. This mixed-dye staining method reduces the total staining and destaining time to less than an hour, and increases the sensitivity to 25 ng of bovine serum albumin, which is greater than the 100 ng sensitivity limit of Coomassie Brilliant Blue R-250 (CBBR) staining. Topics: Azo Compounds; beta-Galactosidase; Carbonic Anhydrases; Coloring Agents; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Myosins; Naphthols; Ovalbumin; Phosphorylase b; Proteins; Rhodamines; Serum Albumin, Bovine; Staining and Labeling	1998

Article

Year

Mixed-dye staining method for protein detection in polyacrylamide gel electrophoresis using calconcarboxylic acid and rhodamine B.

Electrophoresis, 1998, Volume: 19, Issue:14

We have developed a new mixed-dye protein staining method that is simple, rapid, and sensitive. A freshly prepared mixture of calconcarboxylic acid (NN, 0.02%) and rhodamine B (RB, 0.04%) in 40% methanol/7% acetic acid, was used as a staining solution. RB acts as an auxiliary agent to inhibit the binding of NN to the gel matrix, reducing the background staining and therefore enhancing the protein staining by NN. This mixed-dye staining method reduces the total staining and destaining time to less than an hour, and increases the sensitivity to 25 ng of bovine serum albumin, which is greater than the 100 ng sensitivity limit of Coomassie Brilliant Blue R-250 (CBBR) staining.

Topics: Azo Compounds; beta-Galactosidase; Carbonic Anhydrases; Coloring Agents; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Myosins; Naphthols; Ovalbumin; Phosphorylase b; Proteins; Rhodamines; Serum Albumin, Bovine; Staining and Labeling

1998