ovalbumin and boric-acid

The hollow MCM-48 polyethyleneimine carboxyphenylboronic acid molecularly imprinted polymers (H-MPC@MIPs) were synthesized to efficiently and selectively separate and enrich the ovalbumin (OVA) in egg white samples. Polyethyleneimine contained enough active amino groups to increase the amount of boric acid molecules modified to silica nanoparticles. Meanwhile, the materials were etched to enhance the adsorption effect. The H-MPC@MIPs exhibited a rapid adsorption equilibrium rate (within 30 min) and outstanding adsorption capacity for OVA (1334.1 mg g

Topics: Adsorption; Molecular Imprinting; Nanoparticles; Ovalbumin; Polyethyleneimine; Polymers

Glycoproteins play extraordinary roles in biology and clinic. The specifically sensitive detection of glycoproteins by electrochemical methods is still a challenging task due to their poor electro-activity and sensitive nature to environment. In this work, ovalbumin (OVA), a model glycoprotein, was sensitively detected by a molecularly imprinted polymer (MIP) based electrochemical sensor, which was prepared by electropolymerizing 3-thiophene boric acid in the presence of OVA. Due to boronate affinity, the rebound OVA interacted with ferrocene boric acid (Fc-BA) to construct a sandwich structural sensing platform. Dual-recognition elements, imprinted effect and the boronate affinity, enabled the sensor to recognize OVA from other proteins. The rebinding of OVA caused the current changes of thionine and Fc-BA, which were combined as a dual-signal for OVA sensitive detection with a low limit of detection of 0.82 pg/mL (S/N = 3). The good performances of sensor indicated its potential applications in clinical diagnosis and other related fields.

Topics: Boric Acids; Electrochemistry; Glycoproteins; Limit of Detection; Molecular Imprinting; Ovalbumin; Polymers; Thiophenes

To separate the microheterogeneous components of glycoproteins, capillary electrophoresis (CE) conditions were systematically investigated with ovalbumin from chicken egg white as the main testing sample. In addition to the well-known adsorption effect, a reversible capillary wall partitioning effect was found to contribute greatly to the separation and the favorable lubing wall was that coated with polyacrylamide. The separation media, especially the buffer composition, its concentration and pH, were also found to be critical. Buffers composed of boric acid and alcohol amines were demonstrated to be more effective media than gels and other solutions, but the selection of the buffer concentrations and pH was dependent on samples. By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). In contrast, free-solution CE with uncoated capillaries and gel electrophoresis with polyacrylamide-filled capillaries yielded only five or less peaks.

Topics: Acrylic Resins; Animals; Boric Acids; Buffers; Chickens; Electrophoresis, Capillary; Glycoproteins; Hydrogen-Ion Concentration; Ovalbumin