ovalbumin and 2-2--(hydroxynitrosohydrazono)bis-ethanamine

ovalbumin has been researched along with 2-2--(hydroxynitrosohydrazono)bis-ethanamine* in 1 studies

Other Studies

1 other study(ies) available for ovalbumin and 2-2--(hydroxynitrosohydrazono)bis-ethanamine

ArticleYear
Suppressive effects of nitric oxide-releasing prednisolone NCX-1015 on the allergic pleural eosinophil recruitment in rats.
    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2008, Volume: 38, Issue:11

    The addition of a nitric oxide (NO)-releasing moiety to prednisolone was shown to enhance the anti-inflammatory activity of this glucocorticoid in some experimental conditions, but its effectiveness in the context of eosinophilic inflammation remains to be elucidated.. This study compared the anti-inflammatory effect of prednisolone to a NO-releasing derivative of prednisolone, NCX-1015, using a model of allergen-evoked eosinophil recruitment in rats. The efficacy of a NO-donor compound, DETA-NONOate, was also assessed for comparison.. Wistar rats were actively sensitized with Al(OH)(3) plus ovalbumin and 14 days later challenged with antigen intrapleurally. Treatments were performed locally 1 h before challenge. Cysteinyl-leucotrienes (Cys-LT) and eotaxin were measured by ELISA.. Antigen challenge induced an eosinophil infiltration at 12 h, maximal at 24 h. It also caused an increase in the levels of Cys-LTs in the pleural exudate and in the expression of 5-lipoxygenase (5-LO) in infiltrated leucocytes at 6 h, peaking at 12 h and persisting for at least 24 h. Treatment with equimolar doses of prednisolone and NCX-1015 inhibited the late eosinophil infiltration, although the dose required to produce maximal inhibition was about one-tenth that of prednisolone. Cys-LT generation and 5-LO expression were inhibited by NCX-1015 but not by prednisolone. Treatment with prednisolone combined with the NO-donor DETA-NONOate led to a greater inhibition of the eosinophilia and Cys-LT generation as compared with either drug alone. Administration of the steroid receptor antagonist RU 486, 1 h before prednisolone and NCX-1015, abolished the inhibitory effect of the former, under conditions where it only partially affected the latter.. Our findings indicate that NCX-1015 provided a greater anti-inflammatory effect than prednisolone on the allergic eosinophil recruitment in rats, suggesting that NO-releasing steroids can be considered as a promising therapeutic approach to allergic diseases.

    Topics: Animals; Anti-Inflammatory Agents; Arachidonate 5-Lipoxygenase; Chemokine CCL11; Cysteine; Disease Models, Animal; Drug Therapy, Combination; Eosinophilia; Eosinophils; Hypersensitivity; Leukocytes; Leukocytes, Mononuclear; Leukotrienes; Male; Mifepristone; Neutrophils; Nitric Oxide Donors; Nitroso Compounds; Ovalbumin; Pleural Cavity; Pleurisy; Prednisolone; Rats; Rats, Wistar; Receptors, Glucocorticoid

2008