osteogenic-growth-peptide and titanium-dioxide

To investigate the influence of surface-biofunctionalized substrates on osteoblast behavior, a layer of aligned TiO

Topics: Alkaline Phosphatase; Animals; Animals, Newborn; Cell Differentiation; Cell Proliferation; Cell Survival; Gene Expression Regulation; Histones; Intercellular Signaling Peptides and Proteins; Microscopy, Atomic Force; Microscopy, Fluorescence; Nanotubes; Osteoblasts; Osteogenesis; Photoelectron Spectroscopy; Rats; RNA, Messenger; Surface Properties; Titanium

A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO2, ZrO2, CeO2, Fe3O4 and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO2-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein (OCN) markers were upregulated 3-fold and 60-fold, respectively, relative to controls at 21 days. Similarly, 3-fold more calcium was deposited using the OGP tethered dendron compared to TiO2. These catechol-bearing dendrons provide a fast and efficient method to functionalize a wide range of inorganic materials with bioactive peptides and have the potential to be used in coating orthopaedic implants and fixation devices.

Topics: 3T3 Cells; Adsorption; Alkaline Phosphatase; Animals; Catechols; Cell Adhesion; Cell Differentiation; Cell Proliferation; Dendrimers; Histones; Hydrogen-Ion Concentration; Immobilized Proteins; Intercellular Signaling Peptides and Proteins; Mice; Minerals; Osteogenesis; Surface Properties; Titanium