orientin and protocatechuic-acid

To establish the qualitative and quantitative detective methods of Herba Polygoni Orientalis.. Isorientin, orientin, protocatechuic acid and gallic acid in Herba Polygoni Orientalis were identified by TLC. The contents of isorientin and orientin in Herba Polygoni Orientalis were determined by HPLC.. Isorientin, orientin, protocatechuic acid and gallic acid could be identified by TLC. Isorientin and orientin were well separated with Diamonsil C18 column and acetonitrile-0.1% phosphoric acid (18:82) as mobile phase. The linear range of isorientin was 0.075-0.90 microg. The average recovery of isorientin was 98.8% and RSD was 2.1%. The linear range of orientin was 0.041-0.49 microg. The average recovery of orientin was 98.8% and RSD was 2.1%.. The methods can be used for qualitative identification and quantitation determination of Herba Polygoni Orientalis.

Topics: Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Flavonoids; Gallic Acid; Glucosides; Hydroxybenzoates; Luteolin; Plants, Medicinal; Polygonum; Quality Control; Reproducibility of Results