oregon-green-488-carboxylic-acid and 2-chloro-1-methylpyridinium

oregon-green-488-carboxylic-acid has been researched along with 2-chloro-1-methylpyridinium* in 1 studies

Other Studies

1 other study(ies) available for oregon-green-488-carboxylic-acid and 2-chloro-1-methylpyridinium

ArticleYear
Fluorescent high-performance liquid chromatography assay for lipophilic alcohols.
    Analytical biochemistry, 2011, Dec-01, Volume: 419, Issue:1

    A new ultrasensitive fluorescent derivatization procedure for chromatographic analysis of primary, secondary, and nonpolar tertiary alcohols is described. The procedure uses Bodipy FL in basic dichloromethane solution with Mukaiyama's reagent (2-chloro-1-methylpyridinium iodide) to form highly fluorescent ester derivatives that can be separated by silica normal-phase high-performance liquid chromatography (HPLC). Rhodamine WT and Oregon green 488 were also useful derivatization reagents. The detection limit for detection of cholesterol and bryostatin by Bodipy FL was less than 1fmol. The reaction conditions are gentle enough that low concentrations of unstable alcohols such as bryostatin 1 can be measured.

    Topics: Alcohols; Anthracenes; Bryostatins; Candida; Carboxylic Acids; Cholesterol; Chromatography, High Pressure Liquid; Coumarins; Eosine Yellowish-(YS); Esterification; Fluorescein-5-isothiocyanate; Fluorescence; Hydrazines; Indicators and Reagents; Lipase; Pyridinium Compounds; Rhodamines; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization

2011