orabase and caffeic-acid

Cassava starch/sodium carboxymethyl cellulose (CC) was used as the substrate to create a multipurpose food packaging film, and caffeic acid@silica nanoparticles (C@SNPs) was added. The encapsulation rate of caffeic acid in C@SNPs was 84.7 ± 0.97 %. According to SEM pictures, the nanoparticles were evenly dispersed throughout the film and exhibited good compatibility with the other polymers. C@SNPs was added, which enhanced the physical characteristics of film and decreased its water solubility. The best mechanical and oxygen barrier qualities among them are found in the C@SCC

Topics: Anti-Bacterial Agents; Carboxymethylcellulose Sodium; Escherichia coli O157; Food Packaging; Manihot; Meat; Nanoparticles; Permeability; Sodium; Staphylococcus aureus; Starch

Aromatic compounds, such as p-coumaric acid (p-CA) and caffeic acid, are secondary metabolites of various plants, and are widely used in diet and industry for their biological activities. In addition to expensive and unsustainable methods of plant extraction and chemical synthesis, the strategy for heterologous synthesis of aromatic compounds in microorganisms has received much attention. As the most abundant renewable resource in the world, lignocellulose is an economical and environmentally friendly alternative to edible, high-cost carbon sources such as glucose.. In the present study, carboxymethyl-cellulose (CMC) was utilized as the sole carbon source, and a metabolically engineered Saccharomyces cerevisiae strain SK10-3 was co-cultured with other recombinant S. cerevisiae strains to achieve the bioconversion of value-added products from CMC. By optimizing the inoculation ratio, interval time, and carbon source content, the final titer of p-CA in 30 g/L CMC medium was increased to 71.71 mg/L, which was 155.9-fold higher than that achieved in mono-culture. The de novo biosynthesis of caffeic acid in the CMC medium was also achieved through a three-strain co-cultivation. Caffeic acid production was up to 16.91 mg/L after optimizing the inoculation ratio of these strains.. De novo biosynthesis of p-CA and caffeic acid from lignocellulose through a co-cultivation strategy was achieved for the first time. This study provides favorable support for the biosynthesis of more high value-added products from economical substrates. In addition, the multi-strain co-culture strategy can effectively improve the final titer of the target products, which has high application potential in the field of industrial production.

Topics: Caffeic Acids; Carbon; Carboxymethylcellulose Sodium; Coculture Techniques; Coumaric Acids; Culture Media; Escherichia coli; Metabolic Engineering; Saccharomyces cerevisiae