orabase and 9-10-phenanthrenequinone

This paper describes a diffusion-controlled electrolysis of glucose with mediated bioelectrocatalysis at microdisk-electrodes. Under conditions of an extremely fast enzyme reaction, compared with the diffusion of glucose, the current in potential-step chronoamperometry reaches an almost steady state within 10 s, and is proportional to the glucose concentration. The current can be detected at +0.1 V (vs. Ag|AgCl) with 9,10-phenanthrenequinone as a mediator. The addition of carboxymethylcellulose increased the linear range up to 10 mM.

Topics: Aspergillus; Biocatalysis; Biosensing Techniques; Carboxymethylcellulose Sodium; Diffusion; Electrolysis; Electron Transport; Flavin-Adenine Dinucleotide; Glucose; Glucose 1-Dehydrogenase; Microelectrodes; Phenanthrenes