oligomycins and dibromobimane

oligomycins has been researched along with dibromobimane* in 1 studies

Other Studies

1 other study(ies) available for oligomycins and dibromobimane

Article	Year
Bromobimane crosslinking studies in oligomycin-sensitive ATPase from beef heart mitochondria. Mr 31 000 protein crosslinked. FEBS letters, 1982, Dec-13, Volume: 150, Issue:1 Using a bromobimane fluorescent label the Mr 31 000 protein band oligomycin-sensitive (OS)-ATPase from beef heart mitochondria is shown to become much intensified by 2-mercaptopropionylglycine. In the presence of 3.5 nmol/mg protein of the thiol reagent ATP-Pi exchange activity is increased by 90%. With the fluorescent crosslinking reagent dibromobimane (DB) we show that a new fluorescent peak appears between Mr 50 000 and 60 000. ATP-Pi exchange is very much decreased by DB. The results suggest that for regulation of ATP-synthetase activity sulfhydryl groups in the region of the Mr 31 000 protein(s) play an important role. Topics: Adenosine Triphosphatases; Animals; Bridged Bicyclo Compounds; Bridged-Ring Compounds; Cattle; Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Mitochondria, Heart; Molecular Weight; Oligomycins	1982

Article

Year

Bromobimane crosslinking studies in oligomycin-sensitive ATPase from beef heart mitochondria. Mr 31 000 protein crosslinked.

FEBS letters, 1982, Dec-13, Volume: 150, Issue:1

Using a bromobimane fluorescent label the Mr 31 000 protein band oligomycin-sensitive (OS)-ATPase from beef heart mitochondria is shown to become much intensified by 2-mercaptopropionylglycine. In the presence of 3.5 nmol/mg protein of the thiol reagent ATP-Pi exchange activity is increased by 90%. With the fluorescent crosslinking reagent dibromobimane (DB) we show that a new fluorescent peak appears between Mr 50 000 and 60 000. ATP-Pi exchange is very much decreased by DB. The results suggest that for regulation of ATP-synthetase activity sulfhydryl groups in the region of the Mr 31 000 protein(s) play an important role.

Topics: Adenosine Triphosphatases; Animals; Bridged Bicyclo Compounds; Bridged-Ring Compounds; Cattle; Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Mitochondria, Heart; Molecular Weight; Oligomycins

1982