nuezhenide and wedelolactone

The quantitative analysis of multiple indexes remains an important quality evaluation method of traditional Chinese medicine (TCM) herbal formulas. The Chinese Pharmacopoeia 2015 only stipulates the content of a single component, specnuezhenide, in Erzhiwan composed of the Fructus Ligustri Lucidi (FLL) powder and aqueous extracts of Herba Ecliptae (HE). To generalize the intrinsic quality of Erzhiwan, a novel C

Topics: Antioxidants; Apigenin; Chromatography, High Pressure Liquid; Coumarins; Drugs, Chinese Herbal; Fruit; Glucosides; Glycosides; Isomerism; Ligustrum; Limit of Detection; Oleanolic Acid; Phenols; Pyrans; Reproducibility of Results; Triterpenes; Ursolic Acid

To establish an HPLC method for simultaneous determination of nuzhenide, specnuezhenide, wedelolactone and oleanic acid in Erzhiwan.. The DIKMA C18 (4.6 mm x 200 mm, 5 microm) column was adopted with acetonitrile and 0.1% phosphoric acid solution as the mobile phase and gradient elution. The flow rate was 1.0 mL x min(-1) and the volume of injection was 20 microL. The column temperature was maintained at 30 degrees C and the detective wavelength was set at 215 nm.. There were good linear relationships between the peak area and concentration at the range of 2.008-80.32 (r = 0.999 6), 5.872-234.88 (r = 0.999 7) , 0.9-36 (r = 0.999 9), 13.24-529.6 mg x L(-1) (r = 0.999 6) for nuzhenide, specnuezhenide, wedelolactone and oleanic acid, respectively. The average recovery rates of nuzhenide, specnuezhenide, wedelolactone and oleanic acid were 99.25%, 98.70%, 96.23% and 101.5%, respectively, with RSD of less than 3%.. The established method was so easy, rapid and accurate that it can be used as an effective way for quality control of Erzhiwan.

Topics: Chromatography, High Pressure Liquid; Coumarins; Drugs, Chinese Herbal; Glucosides; Pyrans