nsc-366140 and mifentidine

This minireview addresses the usefulness of nonaqueous capillary electrophoresis-mass spectrometry (NACE-MS), mainly in the analysis of lipophilic peptides such as gramicidin S and bacitracin, and therapeutic drugs such as pyrazoloacridine, the H2-antagonist mifentidine, tamoxifen, and their metabolites. The beneficial effects of NACE-MS in typical bioanalytical applications are analyzed case by case. A suitable and widely applicable NACE-MS analysis is identified, which is an electrolyte buffer containing ammonium acetate (5-50 mM) and/or acetic acid (up to 100 mM) with varying composition of organic solvents. Either acetonitrile or methanol or a mixture of the two are mostly utilized in the nonaqueous media. Primary considerations in developing NACE-MS are also discussed.

Topics: Acridines; Animals; Anti-Bacterial Agents; Bacitracin; Cells, Cultured; Electrophoresis, Capillary; Estrogen Antagonists; Gramicidin; Histamine H2 Antagonists; Imidazoles; Intercalating Agents; Liver; Mass Spectrometry; Mice; Peptides; Pyrazoles; Tamoxifen; Time Factors