novobiocin and 4-hydroxybenzoic-acid

novobiocin has been researched along with 4-hydroxybenzoic-acid* in 2 studies

Other Studies

2 other study(ies) available for novobiocin and 4-hydroxybenzoic-acid

Article	Year
CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis. The Journal of biological chemistry, 2003, Aug-15, Volume: 278, Issue:33 The aminocoumarin antibiotics novobiocin and clorobiocin contain a 3-dimethylallyl-4-hydroxybenzoate (3DMA-4HB) moiety. The biosynthesis of this moiety has now been identified by biochemical and molecular biological studies. CloQ from the clorobiocin biosynthetic gene cluster in Streptomyces roseochromogenes DS 12976 has recently been identified as a 4-hydroxyphenylpyruvate-3-dimethylallyltransferase. In the present study, the enzyme CloR was overexpressed in Escherichia coli, purified, and identified as a bifunctional non-heme iron oxygenase, which converts 3-dimethylallyl-4-hydroxyphenylpyruvate (3DMA-4HPP) via 3-dimethylallyl-4-hydroxymandelic acid (3DMA-4HMA) to 3DMA-4HB by two consecutive oxidative decarboxylation steps. In 18O2 labeling experiments we showed that two oxygen atoms are incorporated into the intermediate 3DMA-4HMA in the first reaction step, but only one further oxygen is incorporated into the final product 3DMA-4HB during the second reaction step. CloR does not show sequence similarity to known oxygenases. It apparently presents a novel member of the diverse family of the non-heme iron (II) and alpha-ketoacid-dependent oxygenases, with 3DMA-4HPP functioning both as an alpha-keto acid and as a hydroxylation substrate. The reaction catalyzed by CloR represents a new pathway for the formation of benzoic acids in nature. Topics: Aminocoumarins; Bacterial Proteins; Benzoates; Coumarins; Gene Expression Regulation, Bacterial; Heme; Iron; Novobiocin; Oxygenases; Parabens; Streptomyces	2003
Calorimetric analysis of microbial growth: with special reference to quantitative evaluation of drug action. The Tokai journal of experimental and clinical medicine, 1990, Volume: 15, Issue:5 Our research method for the calorimetric characterization of the biological effects of drugs and other chemicals on metabolic activities of living cells is outlined. The effects of various substances on different microbial systems were studied quantitatively using a calorimeter, and the results were used to plot drug potency curves for each drug. The method was also used to study microbial activity in soil. It was found to be a useful technique for the quantitative characterization of pollutants in ecological systems. Topics: Anti-Bacterial Agents; Aspergillus oryzae; Body Temperature Regulation; Calorimetry; Dietary Fiber; Escherichia coli; Food Preservatives; Glucose; Microbial Sensitivity Tests; Models, Biological; Novobiocin; Osmolar Concentration; Parabens; Regression Analysis; Soil	1990

Article

Year

CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis.

The Journal of biological chemistry, 2003, Aug-15, Volume: 278, Issue:33

The aminocoumarin antibiotics novobiocin and clorobiocin contain a 3-dimethylallyl-4-hydroxybenzoate (3DMA-4HB) moiety. The biosynthesis of this moiety has now been identified by biochemical and molecular biological studies. CloQ from the clorobiocin biosynthetic gene cluster in Streptomyces roseochromogenes DS 12976 has recently been identified as a 4-hydroxyphenylpyruvate-3-dimethylallyltransferase. In the present study, the enzyme CloR was overexpressed in Escherichia coli, purified, and identified as a bifunctional non-heme iron oxygenase, which converts 3-dimethylallyl-4-hydroxyphenylpyruvate (3DMA-4HPP) via 3-dimethylallyl-4-hydroxymandelic acid (3DMA-4HMA) to 3DMA-4HB by two consecutive oxidative decarboxylation steps. In 18O2 labeling experiments we showed that two oxygen atoms are incorporated into the intermediate 3DMA-4HMA in the first reaction step, but only one further oxygen is incorporated into the final product 3DMA-4HB during the second reaction step. CloR does not show sequence similarity to known oxygenases. It apparently presents a novel member of the diverse family of the non-heme iron (II) and alpha-ketoacid-dependent oxygenases, with 3DMA-4HPP functioning both as an alpha-keto acid and as a hydroxylation substrate. The reaction catalyzed by CloR represents a new pathway for the formation of benzoic acids in nature.

Topics: Aminocoumarins; Bacterial Proteins; Benzoates; Coumarins; Gene Expression Regulation, Bacterial; Heme; Iron; Novobiocin; Oxygenases; Parabens; Streptomyces

2003

Calorimetric analysis of microbial growth: with special reference to quantitative evaluation of drug action.

The Tokai journal of experimental and clinical medicine, 1990, Volume: 15, Issue:5

Our research method for the calorimetric characterization of the biological effects of drugs and other chemicals on metabolic activities of living cells is outlined. The effects of various substances on different microbial systems were studied quantitatively using a calorimeter, and the results were used to plot drug potency curves for each drug. The method was also used to study microbial activity in soil. It was found to be a useful technique for the quantitative characterization of pollutants in ecological systems.

Topics: Anti-Bacterial Agents; Aspergillus oryzae; Body Temperature Regulation; Calorimetry; Dietary Fiber; Escherichia coli; Food Preservatives; Glucose; Microbial Sensitivity Tests; Models, Biological; Novobiocin; Osmolar Concentration; Parabens; Regression Analysis; Soil

1990