noc-9 and 4-5-diaminofluorescein

noc-9 has been researched along with 4-5-diaminofluorescein* in 1 studies

Other Studies

1 other study(ies) available for noc-9 and 4-5-diaminofluorescein

Article	Year
Reliable in vitro measurement of nitric oxide released from endothelial cells using low concentrations of the fluorescent probe 4,5-diaminofluorescein. FEBS letters, 2001, Oct-05, Volume: 506, Issue:2 4,5-Diaminofluorescein (DAF-2) and its membrane-permeable derivate DAF-2 diacetate are fluorescent probes that have been developed to perform real-time biological detection of nitric oxide (NO). Their use for intracellular imaging, however, has recently been seriously questioned and data using DAF-2 for extracellular NO detection at low levels, as for example released from endothelial cells, are rare. Here we show that a reliable detection of low levels of NO in biological systems by DAF-2 is possible (a) by using low DAF-2 concentrations (0.1 microM) and (b) by subtracting the DAF-2 auto-fluorescence from the measured total fluorescence. The described method allows easy real-time detection of endothelial NO formation. Topics: Cell Line; Endothelium, Vascular; Enzyme Inhibitors; Fluorescein; Fluorescent Dyes; Humans; Molecular Structure; Nitric Oxide; omega-N-Methylarginine; Spectrometry, Fluorescence; Tetradecanoylphorbol Acetate; Triazenes	2001

Article

Year

Reliable in vitro measurement of nitric oxide released from endothelial cells using low concentrations of the fluorescent probe 4,5-diaminofluorescein.

FEBS letters, 2001, Oct-05, Volume: 506, Issue:2

4,5-Diaminofluorescein (DAF-2) and its membrane-permeable derivate DAF-2 diacetate are fluorescent probes that have been developed to perform real-time biological detection of nitric oxide (NO). Their use for intracellular imaging, however, has recently been seriously questioned and data using DAF-2 for extracellular NO detection at low levels, as for example released from endothelial cells, are rare. Here we show that a reliable detection of low levels of NO in biological systems by DAF-2 is possible (a) by using low DAF-2 concentrations (0.1 microM) and (b) by subtracting the DAF-2 auto-fluorescence from the measured total fluorescence. The described method allows easy real-time detection of endothelial NO formation.

Topics: Cell Line; Endothelium, Vascular; Enzyme Inhibitors; Fluorescein; Fluorescent Dyes; Humans; Molecular Structure; Nitric Oxide; omega-N-Methylarginine; Spectrometry, Fluorescence; Tetradecanoylphorbol Acetate; Triazenes

2001