nk-2761 and diacetylmonoxime

Multiple-site optical recording of transmembrane voltage activity (MSORTV), using a voltage-sensitive dye and a 12 x 12-element photodiode array, was employed to monitor action potentials in the rat atrium. Atrial preparations including the sinus node area, caval area, and atrial septum were dissected from adult rat hearts and stained with a voltage-sensitive merocyanine-rhodanine dye (NK2761). For suppression of optical artefacts due to contractile movements, a bathing medium containing 2,3-butanedione monoxime (BDM: 10-20 mM) was used. The spread of spontaneous excitation from the pacemaker was assessed optically by timing the initiation of the action potential-related extrinsic absorption changes. The optical signals were recorded from more than 300 contiguous loci in the atrium by sliding the photodiode array over the image of the preparation; in this way, the intra-atrial conduction pattern of spontaneous excitation was mapped. The obtained maps revealed a non-radial spread of excitatory waves originating in the pacemaking area over the atrium. Furthermore, we also mapped the conduction pattern of long-lasting tachycardia-like excitation evoked by a short train of electrical stimulation (5-10 Hz, 1-2s) applied with a bipolar electrode. These maps suggest that excitatory waves are propagated in a circular pathway which often surrounds the ostium of the superior or inferior vena cava. Various patterns for the pathway were also demonstrated optically.

Topics: Action Potentials; Animals; Atrial Function; Cholinesterase Reactivators; Coloring Agents; Diacetyl; Electric Stimulation; Electrophysiology; Female; Heart Conduction System; Image Processing, Computer-Assisted; In Vitro Techniques; Male; Myocardial Contraction; Organ Preservation Solutions; Rats; Rats, Wistar; Rhodanine; Tachycardia; Thiazolidines