nivalenol and acetonitrile

nivalenol has been researched along with acetonitrile* in 2 studies

Other Studies

2 other study(ies) available for nivalenol and acetonitrile

ArticleYear
Development, optimization and validation of a multimethod for the determination of 36 mycotoxins in wines by liquid chromatography-tandem mass spectrometry.
    Talanta, 2014, Volume: 129

    A fast and efficient multimethod for the determination of 36 mycotoxins in wine, using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), was developed, optimized, validated and implemented in routine analysis. A simplified, quick extraction was performed with acetonitrile, derived from the QuEChERS (quick, easy, cheap, effective, rugged and safe) approach, which was traditionally developed for pesticides analysis. This study aimed at a single extraction and chromatographic separation for 36 mycotoxins. Optimization tests were performed to find the proper ratio of wine: water and extraction solvent and the need for an additional buffering step with ammonium formate/formic acid and a dispersive SPE cleanup with various sorbents. The dSPE steps did not show significant improvement in analysis results, therefore, it was not applied in the final method to be validated. The mycotoxins were separated and detected on a UPLC-MS/MS system, used in the ESI positive ionization mode. The various mycotoxins were divided in three different concentration level groups, according to their sensitivity in UPLC-MS/MS. The validation was performed by analyzing recovery samples at three different spike levels with six replicates (n=6) at each level. Linearity (r(2)) of calibration curves, accuracy (recovery %), instrument limits of detection and method limits of quantification (LOD and LOQ), precision (RSD%) and matrix effects (%) were determined for each individual mycotoxin. From the 36 mycotoxins analyzed by UPLC-MS/MS (ESI+), 35 showed average recoveries in the range 70-120%, and 86% of these with a RSD≤20% at the lowest spike level (for Group I, II and III, respectively, 1, 50 and 10 µg kg(-1)). The higher spike levels showed even better results. Only nivalenol could not be quantified at any concentration level. The method LOQ for 86% of the mycotoxins studied was the lowest spike level tested. The matrix effect observed was low for most mycotoxins analyzed and had no significant influence on the analytical results obtained. The developed procedure was applied successfully in routine analysis in a survey of wine samples originating from different countries.

    Topics: Acetonitriles; Buffers; Calibration; Chromatography, Liquid; Cost-Benefit Analysis; Food Analysis; Indoles; Lactones; Limit of Detection; Mycophenolic Acid; Mycotoxins; Ochratoxins; Pesticides; Reproducibility of Results; Solvents; Tandem Mass Spectrometry; Trichothecenes; Water; Wine; Zearalenone

2014
Effect of time, temperature and solvent on the stability of T-2 toxin, HT-2 toxin, deoxynivalenol and nivalenol calibrants.
    Food additives and contaminants, 2001, Volume: 18, Issue:11

    The influence of solvent, storage time and temperature on the stability of the trichothecene mycotoxins T-2 toxin (T-2), HT-2 toxin (HT-2), deoxynivalenol (DON) and nivalenol (NIV) was investigated. Toxins in acetonitrile, ethyl acetate or as thin film were stored in sealed glass ampoules at -18, 4, 25 and 40 degrees C for up to 24 months. Samples were analysed by HPLC with UV detection. The results should that acetonitrile was the preferred solvent and no significant (t0.95-test) decomposition occurred for any of the four trichothecenes when stored for 24 months at 25 degrees C or 3 months at 40 degrees C. T-2 and HT-2 in ethyl acetate or as thin film were also stable under the same conditions. DON and NIV in ethyl acetate or as thin film were stable for up to 24 months at -18 degrees C, but a significant decomposition of DON and NIV in ethyl acetate was observed for both toxins after 24 months of storage at 4 degrees C and after 12 months at 25 degrees C. When stored as thin film, a significant trend of decomposition of DON occurred after 24 months of storage at 4 degrees C and after 6 months of storage at 25 degrees C. A significant decrease of NIV stored as thin filmn was observed after 9 months at 25 degrees C. In conclusion, acetonitrile was the most suitable solvent for long-term storage of T-2, HT-2, DON and NIV.

    Topics: Acetates; Acetonitriles; Chromatography, High Pressure Liquid; Humans; Linear Models; Mycotoxins; Solvents; T-2 Toxin; Temperature; Time Factors; Trichothecenes

2001