nitryl-chloride has been researched along with 3-nitrotyrosine* in 1 studies
1 other study(ies) available for nitryl-chloride and 3-nitrotyrosine
| Article | Year |
|---|---|
Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrometry.
Reaction of reactive nitrogen species (RNS), such as peroxynitrite and nitryl chloride with soluble tyrosine and tyrosine residues in proteins produces soluble 3-nitro-tyrosine and 3-nitro-tyrosino-proteins, respectively. Regular proteolysis of 3-nitro-tyrosino-proteins yields soluble 3-nitro-tyrosine. 3-Nitro-tyrosine circulates in plasma and is excreted in the urine. Both circulating and excretory 3-nitro-tyrosine are considered suitable biomarkers of nitrative stress. Tandem mass spectrometry coupled with gas chromatography (GC-MS/MS) or liquid chromatography (LC-MS/MS) is one of the most reliable analytical techniques to determine 3-nitro-tyrosine. Here, we describe protocols for the quantitative determination of soluble 3-nitro-tyrosine in human plasma and urine by GC-MS/MS. Topics: Gas Chromatography-Mass Spectrometry; Humans; Nitrites; Peroxynitrous Acid; Solubility; Tandem Mass Spectrometry; Tyrosine | 2012 |