nitrophenols and kebuzone

The isotachophoretic (ITP) behaviour and separation of the anti-inflammatory drugs kebuzone (KB), tribuzone (TB) and phenylbutazone (PB) was studied in the operational system of HCl/His (leading electrolyte, LE) and 4-nitrophenol (terminating electrolyte, TE). The effective mobilities were 19.4 x 10(-9) m2 V-1 s-1 for KB, 18.1 x 10(-9) m2 V-1 s-1 for TB and 18.9 x 10(-9) m2 V-1 s-1 for PB when using an optimised system with 10 mM HCl + 40 mM His (pH 6.63) as LE and 10 mM 4-nitrophenol as TE. The calibration graphs were rectilinear (r = 0.9982-0.9996) in the range 20 to 600 mumol 1-1 of KB, TB or PB. The ITP method was used for determining the content of KB, TB or PB in mass-produced pharmaceuticals as tablets, coated tablets, injections, and ointments. The results of the ITP determination were in good agreement with those of standard pharmacopoeial methods.

Topics: Anti-Inflammatory Agents, Non-Steroidal; Electrolytes; Electrophoresis; Hydrochloric Acid; Hydrogen-Ion Concentration; Nitrophenols; Phenylbutazone