nitrophenols and glucose-1-phosphate

This work has concerned the description of a method suitable for the measurement, on living trout, of the intestinal alkaline phosphatase activity. The experiments consist in the perfusion, in the lumen, of an appropriate substrate and in the prevention of its absorption or that of the products of its hydrolysis. This condition has not been satisfied by using paranitrophenyl phosphate which has been replaced by glucose-1-phosphate. The absorption of glucose produced has been inhibited by phloridzin (10(-4) M) in a KCl (150 mM) solution. Under these conditions an hydrolysis of glucose phosphate has been shown at alkaline pH, which remains linear with time up to 60 min. A research of the Km of the enzyme activity has been carried out.

Topics: Alkaline Phosphatase; Animals; Glucose; Glucosephosphates; Hydrogen-Ion Concentration; Intestinal Absorption; Intestines; Kinetics; Microvilli; Nitrophenols; Organophosphorus Compounds; Phlorhizin; Salmonidae; Trout