nitrophenols and 4-isopropylphenol

Octolig®, a polyethylenediimine ligand covalently attached to high-surface area silica gel, was used to study the removal of phenolic compounds from aqueous samples by column chromatography. Model phenolic compounds of Bisphenol A (BPA), 4-isopropylphenol and 4-(t-butyl) phenol, were selected for this study due to their similarities in pKa and log P values. The percent removal of these compounds by Octolig® was 26 ± 2 and 22 ± 2, respectively. Furthermore, the three isomers of nitrophenol were investigated as well as additional phenolic compounds, such as amoxicillin and five phenolic dyes. These compounds have a pKa range of 2-10.2. The compounds that have pKa values less than 8.3 were able to be completely removed by Octolig®, yet compounds with pKa values of 8.3 and higher resulted in approximately 20-26% removal.

Topics: Benzhydryl Compounds; Chemical Precipitation; Chromatography; Environmental Pollutants; Environmental Pollution; Ligands; Nitrophenols; Phenols; Polyethylenes; Silica Gel

Mouse nasal cytosols show high sulfotransferase (ST) activities toward phenolic aromatic odorants, but have little activities for most alcoholic aromatic odorants. Most ST activities toward the phenolic odorants preferred slightly acidic pH (6.4) and were sensitive to 2,6-dichloro-4-nitrophenol, a specific inhibitor for phenol ST (P-ST) but were not inhibited by triethylamine and tetra-n-butylammonium chloride, which are specific inhibitors for hydroxysteroid ST (HS-ST). These results suggested that P-ST activities are responsible for sulfation of the phenolic odorants. The spectra of the ST activities for these odorants were similar in mouse nasal and liver cytosols, however, nasal cytosols showed much higher ST activity toward cinnamyl alcohol than liver cytosols. This activity preferred higher pH (7.4) compared to the phenolic odorant-ST activities and was inhibited by both types of inhibitors, specific for P-ST and HS-ST. These results appear to indicate the participation of multiple ST isoforms for the sulfation of odorants in mouse nasal cytosols. The existence of P-ST(s) active for the phenolic odorants in olfactory cytosols suggests a role in odorant perception, in particular, in the signal termination process.

Topics: Alcohols; Animals; Arylsulfotransferase; Cytosol; Enzyme Inhibitors; Guaiacol; Hydrogen-Ion Concentration; Isoenzymes; Liver; Male; Mice; Mice, Inbred Strains; Molecular Structure; Naphthols; Nitrophenols; Odorants; Olfactory Mucosa; Phenols; Smell; Substrate Specificity; Sulfotransferases