nitrogenase and catechol

Azotobacter vinelandii is a terrestrial diazotroph well studied for its siderophore production capacity and its role as a model nitrogen fixer. In addition to Fe, A. vinelandii siderophores are used for the acquisition of the nitrogenase co-factors Mo and V. However, regulation of siderophore production by Mo- and V-limitation has been difficult to confirm and knowledge of the full suite of siderophores synthesized by this organism has only recently become available. Using this new information, we conducted an extensive study of siderophore production in N

Topics: Azotobacter vinelandii; Catechols; Citrates; Iron; Molybdenum; Nitrogen; Nitrogen Fixation; Nitrogenase; Pyrrolidinones; Siderophores; Vanadium

Azotobacter chroococcum MH1 was grown in a mannitol and nitrogen free medium supplemented with p-hydroxybenzoic acid, resorcinol, catechol or vanillic acid as a sole carbon source. Growth and nitrogenase activity of p-hydroxybenzoic acid were supported by 8, 6 and 4 mM of p-hydroxybenzoic acid, resorcinol and catechol, respectively. The generation time of 1.71 h in p-hydroxybenzoic acid did not differ from a generation time of 1.64 h, when grown in mannitol. The compound p-hydroxybenzoic acid was utilized rapidly. However, the decomposition of other phenolic compounds tested proceeded slowly. These results suggested that phenolic compounds released during biodegradation of plant wastes could be utilized as carbon sources for both growth and nitrogen fixation of Azotobacter chroococcum.

Topics: Azotobacter; Bacterial Proteins; Biodegradation, Environmental; Catechols; Cell Division; Nitrogenase; Parabens; Phenols; Resorcinols; Soil Microbiology; Vanillic Acid